1887

Abstract

A Gram-staining-negative, non-motile, aerobic bacterium, designated strain A8, was isolated from the beach soil of Muchangpo, Korea. Cells were rod-shaped (0.5–0.6×0.7–1.3 µm) and colonies were colourless, circular with entire edges and had a glistening surface. The isolate grew optimally at 25–35 °C and did not require NaCl for growth. Strain A8 could not assimilate acetate, -lactate, succinate, antipyrine or chloridazon, but weakly assimilated -phenylalanine. Major fatty acids were summed feature 7 (comprising Cω7/ω9/ω12), C and summed feature 4 (comprising Cω7/ iso-C 2-OH). The major isoprenoid quinone was ubiquinone-10 and the DNA G+C content was 72.3 mol%. Comparative 16S rRNA gene sequence studies showed that strain A8 belonged to the family class and was most closely related to type strains of members of the genus (95.7–97.1 % similarity). Signature nucleotides and phylogenetic analysis of the 16S rRNA gene sequence also suggested that strain A8 was affiliated with the genus . Low DNA–DNA relatedness values (3.0±1.8–11.5±3.2 %) indicated that strain A8 represented a distinct species that was separated from other type strains in the genus . On the basis of evidence from a polyphasic study, it is proposed that strain A8 ( = KACC 15042  = LMG 25973) represents the type strain of a novel species, sp. nov. An emended description of the genus is also presented.

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2012-04-01
2019-10-23
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References

  1. Abraham W. R., Macedo A. J., Lünsdorf H., Fischer R., Pawelczyk S., Smit J., Vancanneyt M.. ( 2008;). Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium. . Int J Syst Evol Microbiol 58:, 1939–1949. [CrossRef][PubMed]
    [Google Scholar]
  2. Aslam Z., Im W. T., Ten L. N., Lee S. T.. ( 2005;). Phenylobacterium koreense sp. nov., isolated from South Korea. . Int J Syst Evol Microbiol 55:, 2001–2005. [CrossRef][PubMed]
    [Google Scholar]
  3. Blaschek H. P., Klacik M. A.. ( 1984;). Role of DNase in recovery of plasmid DNA from Clostridium perfringens. . Appl Environ Microbiol 48:, 178–181.[PubMed]
    [Google Scholar]
  4. Buck J. D.. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 2005;). phylip (phylogeny inference package), version 3.67. Distributed by the author.. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  9. Hiraishi A., Ueda Y., Ishihara J., Mori T.. ( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42:, 457–469. [CrossRef]
    [Google Scholar]
  10. Kanso S., Patel B. K.. ( 2004;). Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus Phenylobacterium. . Int J Syst Evol Microbiol 54:, 2141–2146. [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  13. Kumar S., Tamura K., Nei M.. ( 2004;). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef][PubMed]
    [Google Scholar]
  14. Lingens F., Blecher R., Blecher H., Blobel F., Eberspächer J., Fröhner C., Görisch H., Görisch H., Layh G.. ( 1985;). Phenylobacterium immobile gen. nov., sp. nov., a gram-negative bacterium that degrades the herbicide chloridazon. . Int J Syst Bacteriol 35:, 26–39. [CrossRef]
    [Google Scholar]
  15. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Oh Y. S., Lim H. J., Cha I. T., Im W. T., Yoo J. S., Kang U. G., Rhee S. K., Roh D. H.. ( 2009;). Roseovarius halotolerans sp. nov., isolated from deep seawater. . Int J Syst Evol Microbiol 59:, 2718–2723. [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  20. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Tiago I., Mendes V., Pires C., Morais P. V., Veríssimo A.. ( 2005;). Phenylobacterium falsum sp. nov., an alphaproteobacterium isolated from a nonsaline alkaline groundwater, and emended description of the genus Phenylobacterium. . Syst Appl Microbiol 28:, 295–302. [CrossRef][PubMed]
    [Google Scholar]
  23. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  24. Weon H. Y., Kim B. Y., Kwon S. W., Go S. J., Koo B. S., Stackebrandt E.. ( 2008;). Phenylobacterium composti sp. nov., isolated from cotton waste compost in Korea. . Int J Syst Evol Microbiol 58:, 2301–2304. [CrossRef][PubMed]
    [Google Scholar]
  25. Zhang K., Han W., Zhang R., Xu X., Pan Q., Hu X.. ( 2007;). Phenylobacterium zucineum sp. nov., a facultative intracellular bacterium isolated from a human erythroleukemia cell line K562. . Syst Appl Microbiol 30:, 207–212. [CrossRef][PubMed]
    [Google Scholar]
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