1887

Abstract

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, sp. nov. strain HH, was isolated as a co-culture with JF-1 from anaerobic, freeze-dried granular sludge obtained from an upflow anaerobic sludge bed reactor treating sugar beet wastewater. The bacterium converted propionate to acetate in co-culture with JF-1 or MF, but not in co-culture with AZ. The organism could not be cultured axenically with any of the substrates tested and therefore can be considered as a (the first) true anaerobic syntrophic bacterium. The bacterium contained two distinct 16S rRNA gene sequences, with 96·8 % sequence similarity, which were both expressed during syntrophic growth on propionate as revealed by fluorescent hybridization. The most closely related organisms are LR7.2, a bacterium that transforms phenol into benzoate, and SI, a thermophilic, syntrophic propionate-oxidizing bacterium. Other related species belong to the Gram-positive, sulfate-reducing genus . The type strain of is strain HH (=ATCC BAA-615=DSM 15200).

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2005-07-01
2019-10-22
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