1887

Abstract

A novel coccoid, halotolerant actinobacterium, designated strain YIM 70085, was isolated from a soil sample that was collected in Xinjiang Province, China, and characterized by using a polyphasic approach. Optimum growth temperature was 28 °C and growth occurred optimally in culture media that contained 10 % KCl. The peptidoglycan type was A4, -lys–gly–-Glu. Whole-cell sugars consisted of xylose, mannose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phospholipid, one unknown glycolipid and traces of phosphatidylinositol. Menaquinones were MK-8 (83 %), MK-7 (12 %) and MK-9 (15 %). Predominant fatty acids were i-C (44·29 %), ai-C (35·60 %) and ai-C (9·74 %). The DNA G+C content was 53·5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70085 occupies a branch that is distinct from, although very close to, the family in the suborder . Based on its phenotypic characteristics, phylogenetic position (as determined by 16S rRNA gene sequence analysis) and 16S rDNA signature nucleotide data, it is concluded that the isolate represents a novel member of the suborder , for which the name gen. nov., sp. nov. is proposed. The type strain is YIM 70085 (=CCTCC AA001023=DSM 15476).

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2004-03-01
2019-12-12
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