1887

Abstract

Two whitish yellow, Gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga . The bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) NaCl. The DNA G+C content was 61 mol%. The two isolates exhibited a conspecific DNA–DNA relatedness value of 98 %, indicating that they belong to the same species. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 3637 formed a distinct phyletic lineage in the genus (family , class ) and showed the highest sequence similarity (about 97 %) to . DNA–DNA hybridization experiments demonstrated 45 % binding with the DNA of DSM 20657. Physiological and chemotaxonomic characteristics (-diaminopimelic acid in the peptidoglycan, major cellular fatty acids 15 : 0ai and 17 : 0ai) of the bacteria studied were consistent with the genomic and phylogenetic data. On the basis of the results of this study, a novel species, sp. nov., is proposed. The type strain is KMM 3637 (=DSM 15453=ATCC BAA-809).

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2004-11-01
2020-12-03
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