1887

Abstract

Two whitish yellow, Gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga . The bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) NaCl. The DNA G+C content was 61 mol%. The two isolates exhibited a conspecific DNA–DNA relatedness value of 98 %, indicating that they belong to the same species. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 3637 formed a distinct phyletic lineage in the genus (family , class ) and showed the highest sequence similarity (about 97 %) to . DNA–DNA hybridization experiments demonstrated 45 % binding with the DNA of DSM 20657. Physiological and chemotaxonomic characteristics (-diaminopimelic acid in the peptidoglycan, major cellular fatty acids 15 : 0ai and 17 : 0ai) of the bacteria studied were consistent with the genomic and phylogenetic data. On the basis of the results of this study, a novel species, sp. nov., is proposed. The type strain is KMM 3637 (=DSM 15453=ATCC BAA-809).

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2004-11-01
2020-01-23
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vol. , part 6, pp. 2107 - 2111

Phylogenetic position of sp. nov. KMM 3637 according to 16S rRNA gene sequence analysis, including ‘ ’ and a number of other reference sequences. The topology shown was obtained using the bioNJ algorithm and 1000 bootstrap replications with Kimura’s two-parameter correction for the distances. Bootstrap values (%) are indicated only for branches that were also retrieved by maximum-parsimony and maximum-likelihood (P<0.01) analyses; these branches should be considered as the only robust clusters identified by this analysis.

Cellular fatty acid composition of strains of sp. nov.

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