1887

Abstract

A methanogenic organism, designated strain HB-1, from the domain Archaea was isolated from groundwater sampled from a subsurface Miocene formation located in Horonobe, Hokkaido, Japan. The strain grew on methanol, dimethylamine, trimethylamine, dimethylsulfide and acetate but not on monomethylamine, H/CO, formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-reaction-negative, non-motile, irregular cocci that were 1.4–2.9 µm in diameter and occurred singly or in pairs. The strain grew at 20–42 °C (optimum 37 °C), at pH 6.0–7.75 (optimum pH 7.0–7.25) and in 0–0.35 M NaCl (optimum 0.1 M). The G+C content of the genomic DNA was 41.4 mol%. 16S rRNA gene sequencing revealed that the strain was a member of the genus but that it clearly differed from all recognized species of this genus (93.1–97.9 % sequence similarity). The phenotypic and phylogenetic features of strain HB-1 indicate that it represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HB-1 ( = DSM 21571  = JCM 15518  = NBRC 102577).

Erratum
This article contains a correction applying to the following content:
sp. nov., a methanogenic archaeon isolated from a deep subsurface Miocene formation
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2011-10-01
2019-10-16
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Phase-contrast photomicrograph (a), autofluorescence epifluorescence microscopy photograph (excitation at 400-440 nm, emission at 475 nm) after staining with acridine orange (b), and electron micrograph of negatively stained cells (c) of strain HB-1 .

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Influence of temperature at pH 7.0 (a), pH at 37 °C (b), NaCl concentration at 37 °C and pH 7.0 (c), and Mg concentration at 37 °C and pH 7.0 (d), on the growth rate of strain HB-1 . [PDF](66KB)

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