1887

Abstract

A Gram-positive, molinate-degrading bacterium, strain ON4 (=DSM 13485=LMG 21909), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on -ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family , showing the highest 16S rDNA similarity (∼95 %) to members of the genus and ‘’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2 and the major menaquinone MK-9, which are typical of , but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4 and ‘’ DSM 20419 be classified as two novel genera and species, with the respective names gen. nov., sp. nov. and gen. nov., sp. nov

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02851-0
2004-05-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540783.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02851-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Akimov V. N., Lubitz W., Busse H.-J. 1997; Polyamine distribution in actinomycetes with group B peptidoglycan and species of the genera Brevibacterium , Corynebacterium , and Tsukamurella . Int J Syst Bacteriol 47:270–277 [CrossRef]
    [Google Scholar]
  2. Barreiros L., Nogales B., Manaia C. M., Silva Ferreira A. C., Pieper D. H., Reis M. A., Nunes O. C. 2003; A novel pathway for mineralization of the thiocarbamate herbicide molinate by a defined bacterial mixed culture. Environ Microbiol 5:944–953 [CrossRef]
    [Google Scholar]
  3. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. 2002; Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp.nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov.,sp. nov. Int J Syst Evol Microbiol 52:1441–1454 [CrossRef]
    [Google Scholar]
  4. Carlton B. C., Brown B. J. 1981; Gene mutation. In Manual of Methods for General Microbiology pp  409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  6. Collins M. D., Bradbury J. F. 1992; The genera Agromyces Aureobacterium , Clavibacter , Curtobacterium , and Microbacterium .. In The Prokaryotes , 2nd edn. pp  1355–1368 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  7. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Microbiology pp  21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Evtushenko L. I., Takeuchi M. 2003 The family Microbacteriaceae . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn. release 3.14, 31 July 2003 Edited by M. Dworkin, et al New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  9. Felsenstein J. 1989; phylip – phylogeny inference package. Cladistics 5:164–166
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  12. Manaia C. M., Nunes O. C., Nogales B. 2003; Caenibacterium thermophilum gen. nov., sp. nov. isolated from a thermophilic aerobic digester of municipal sludge. Int J Syst Evol Microbiol 53:1375–1382 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  15. Moreira C., Rainey F. A., Nobre M. F., da Silva M. T., da Costa M. S. 2000; Tepidimonas ignava gen. nov., sp. nov. a new chemolithoheterotrophic and slightly thermophilic member of the β - Proteobacteria . Int J Syst Evol Microbiol 50:735–742 [CrossRef]
    [Google Scholar]
  16. Nogales B., Moore E. R. B., Llobet-Brossa E., Rossello-Mora R., Amann R., Timmis K. N. 2001; Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil. Appl Environ Microbiol 67:1874–1884 [CrossRef]
    [Google Scholar]
  17. Park Y. H., Suzuki K., Yim D. G. 7 other authors 1993; Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 94:307–313
    [Google Scholar]
  18. Rainey F., Weiss N., Prauser H., Stackebrandt E. 1994; Further evidence for the phylogenetic coherence of actinomycetes with group B-peptidoglycan and evidence for the phylogenetic intermixing of the genera Microbacterium and Aureobacterium as determined by 16S rDNA analysis. FEMS Microbiol Lett 118:135–140 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Sasaki J., Chijimatsu M., Suzuki K. 1998; Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov. Int J Syst Bacteriol 48:403–410 [CrossRef]
    [Google Scholar]
  21. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  22. Sheridan P. P., Loveland-Curtze J., Miteva V. I., Brenchley J. E. 2003; Rhodoglobus vestalii gen. nov., sp. nov. a novel psychrophilic organism isolated from an Antarctic Dry Valley lake. Int J Syst Evol Microbiol 53:985–994 [CrossRef]
    [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Microbiology pp  409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  25. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacteriun Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
    [Google Scholar]
  26. Tindall B. J. 1990; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02851-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02851-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error