1887

Abstract

A Gram-positive, molinate-degrading bacterium, strain ON4 (=DSM 13485=LMG 21909), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on -ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family , showing the highest 16S rDNA similarity (∼95 %) to members of the genus and ‘’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2 and the major menaquinone MK-9, which are typical of , but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4 and ‘’ DSM 20419 be classified as two novel genera and species, with the respective names gen. nov., sp. nov. and gen. nov., sp. nov

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2004-05-01
2019-10-15
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vol. , part 3, pp. 783 - 789

Cellular fatty acid composition of strains ON4 and DSM 20419

Transmission electron micrographs of negatively stained cells of strain ON4 (A, B, C) and ' ' DSM 20419 (D, E, F) grown on LB medium for 24 h at 30 °C. Bar, 0·5 µm.

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