1887

Abstract

A Gram-negative bacterium, designated AD8, was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8 was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8 was observed at 15–40 °C (optimum 25–30 °C), at pH 5.0–9.5 (optimum pH 6.0–7.5) and with 1.0–11.0 % (w/v) NaCl (optimum 2.0–4.0 %). The predominant fatty acids were Cω7 and C cyclo ω8. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8 belonged to the genus and was most closely related to CK-I3-6 within the class (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AD8 ( = KACC 13699  = DSM 22011).

Funding
This study was supported by the:
  • Cooperative Research Program for Agriculture Science & Technology Development
  • Rural Development Administration (Award J007451)
  • National Research Foundation of Korea
  • Korean Government (MEST), Republic of Korea (Award 2009-0071880)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028332-0
2011-11-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/11/2595.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028332-0&mimeType=html&fmt=ahah

References

  1. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B.-K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  2. Chung B. S., Ryu S. H., Park M., Jeon Y., Chung Y. R., Jeon C. O. 2007; Hydrogenophaga caeni sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 57:1126–1130 [View Article][PubMed]
    [Google Scholar]
  3. Dai X., Wang B. J., Yang Q. X., Jiao N. Z., Liu S. J. 2007; Yangia pacifica gen. nov., sp. nov., a novel member of the Roseobacter clade from coastal sediment of the East China Sea. Int J Syst Evol Microbiol 56:529–533 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 2002; phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  5. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1138–146 [View Article]
    [Google Scholar]
  6. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. 2004; Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54:93–97 [View Article][PubMed]
    [Google Scholar]
  7. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O. 2011; Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. Int J Syst Evol Microbiol 61:1392–1396 [View Article][PubMed]
    [Google Scholar]
  8. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O. 2011; Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. Int J Syst Evol Microbiol 61:1534–1538 [View Article][PubMed]
    [Google Scholar]
  9. Kim J. M., Le N. T., Chung B. S., Park J. H., Bae J.-W., Madsen E. L., Jeon C. O. 2008; Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74:7313–7320 [View Article][PubMed]
    [Google Scholar]
  10. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O. 2010a; Marinobacterium lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 60:1828–1831 [View Article][PubMed]
    [Google Scholar]
  11. Kim J. M., Jung J. Y., Chae H. B., Park W., Jeon C. O. 2010b; Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 60:2877–2881 [View Article][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  13. Lai Q., Qiao N., Wu C., Sun F., Yuan J., Shao Z. 2010; Stappia indica sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 60:733–736 [View Article]
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  15. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  16. Lee S. H., Shim J. K., Kim J. M., Choi H.-K., Jeon C. O. 2011; Henriciella litoralis sp. nov., isolated from a tidal-flat of the Yellow Sea, and transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61:722–727 [View Article]
    [Google Scholar]
  17. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. 2006; Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44:155–161[PubMed]
    [Google Scholar]
  18. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [View Article][PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Sorokin D. Y., Tourova T. P., Muyzer G. 2005; Citreicella thiooxidans gen. nov., sp. nov., a novel lithoheterotrophic sulfur-oxidizing bacterium from the Black Sea. Syst Appl Microbiol 28:679–687 [View Article][PubMed]
    [Google Scholar]
  21. Sorokin D. Y., Tourova T. P., Muyzer G. 2006; Citreicella thiooxidans gen. nov., sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107.. Int J Syst Evol Microbiol 56:1–6 [CrossRef]
    [Google Scholar]
  22. Stamatakis A., Ott M., Ludwig T. 2005; RAxML-OMP: an efficient program for phylogenetic inference on SMPs. In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005). Lecture Notes in Computer Science 3506288–302 Springer Verlag;
    [Google Scholar]
  23. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.028332-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028332-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error