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Abstract

A Gram-negative bacterium, designated AD8, was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8 was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8 was observed at 15–40 °C (optimum 25–30 °C), at pH 5.0–9.5 (optimum pH 6.0–7.5) and with 1.0–11.0 % (w/v) NaCl (optimum 2.0–4.0 %). The predominant fatty acids were Cω7 and C cyclo ω8. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8 belonged to the genus and was most closely related to CK-I3-6 within the class (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AD8 ( = KACC 13699  = DSM 22011).

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2011-11-01
2020-01-29
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vol. , part 11, pp. 2595 - 2599

Transmission electron micrograph of a cell of sp. nov. AD8 , negatively stained with phosphotungstic acid after growth for 2 days at 30 °C on marine agar. Bar, 0.5 µm.



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