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Abstract

A rod-shaped, endospore-forming, Gram-reaction-positive bacterium, designated strain WPCB018, was isolated from a fresh water sample collected from Woopo wetland, Korea. The isolate was identified as a member of the genus on the basis of phenotypic characteristics and phylogenetic inference based on 16S rRNA gene sequence analysis. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. The diamino acid found in the cell-wall peptidoglycan was diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C (32.2 %), C (20.1 %) and C (18.1 %). The DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WPCB018 belongs to a cluster comprising species of the genus , its closest neighbours being PC-147 (97.5 %) and SAFN-007 (96.2 %). Genomic DNA–DNA hybridizations performed with strain WPCB018 and type strains of the species , , , and showed relatedness values of only 10, 17, 18, 19 and 20 %, respectively. On the basis of phenotypic, molecular and genetic evidence, strain WPCB018 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the novel species is WPCB018 ( = KCTC 13280  = JCM 16350).

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2011-11-01
2019-10-16
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Phylogenetic trees showing the position of strain WPCB018 and related members of the genus based on nearly complete 16S rRNA gene sequences using the Fitch-Margoliash (a), maximum-parsimony (b) and maximum-likelihood (c) methods. Bars, 0.02 (a, c) and 10 (b) substitutions per nucleotide position. [PDF](41KB)

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Scanning electron (a) and transmission electron (b) micrographs of cells of strain WPCB018 . Cells were grown on TSB at 30 °C for 2 days (a) and 1 day (b), respectively.

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Polar lipid profiles of strain WPCB018 (a, d), PC-147 (b, e) and S23 (c, f) after two-dimentional TLC detected with ninhydrin (a, b, c) and zinzade (d, e, f). DPG, diphopharidylglycerol; PE, phosphatidylethanolamine; AL, unknown aminolipid; APL, unknown aminophospholipid.

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Levels of DNA-DNA relatedness between strain WPCB018 and related members of the genus [PDF](24KB)

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