1887

Abstract

A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9, was isolated from soil collected on the Korean peninsula. Strain BR-9 grew optimally at pH 6.0–7.0, at 30 °C and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BR-9 belonged to the genus and clustered with DS-139 and WPCB189. Strain BR-9 exhibited 98.2 and 97.5 % 16S rRNA gene sequence similarity with DS-139 and WPCB189, respectively, and <96.7 % sequence similarity with the type strains of other species in the genus . Strain BR-9 contained MK-7 as the predominant menaquinone and iso-C and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acids. The DNA G+C content of strain BR-9 was 38.5 mol%. DNA–DNA relatedness between strain BR-9 and DS-139 and KCTC 12536 was 3.4–4.2 %, which indicated that the isolate was genetically distinct from these type strains. Strain BR-9 was also distinguishable by differences in phenotypic properties. On the basis of the data presented, strain BR-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BR-9 ( = KCTC 23344  = CCUG 60024).

Funding
This study was supported by the:
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award 11-2008-00-002-00)
  • , Program for Collection, Management and Utilization of Biological Resources , (Award M10867010003)
  • , Ministry of Education, Science and Technology (MEST), Republic of Korea
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2012-01-01
2020-07-07
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References

  1. An D.-S., Kim S.-G., Ten L. N., Cho C.-H. 2009; Pedobacter daechungensis sp. nov., from freshwater lake sediment in South Korea. Int J Syst Evol Microbiol 59:69–72 [CrossRef][PubMed]
    [Google Scholar]
  2. Baik K. S., Park Y.-D., Kim M. S., Park S. C., Moon E. Y., Rhee M. S., Choi J. H., Seong C. N. 2007; Pedobacter koreensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57:2079–2083 [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [CrossRef]
    [Google Scholar]
  7. Euzéby J. P. 2010 List of prokaryotic names with standing in nomenclature. Last full update 5 August 2010. http://www.bacterio.cict.fr/p/pedobacter.html
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  9. Hwang C. Y., Choi D. H., Cho B. C. 2006; Pedobacter roseus sp. nov., isolated from a hypertrophic pond, and emended description of the genus Pedobacter . Int J Syst Evol Microbiol 56:1831–1836 [CrossRef][PubMed]
    [Google Scholar]
  10. Jeon Y., Kim J. M., Park J. H., Lee S. H., Seong C.-N., Lee S.-S., Jeon C. O. 2009; Pedobacter oryzae sp. nov., isolated from rice paddy soil. Int J Syst Evol Microbiol 59:2491–2495 [CrossRef][PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  12. Kwon S.-W., Kim B.-Y., Lee K.-H., Jang K.-Y., Seok S.-J., Kwon J.-S., Kim W.-G., Weon H.-Y. 2007; Pedobacter suwonensis sp. nov., isolated from the rhizosphere of Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 57:480–484 [CrossRef][PubMed]
    [Google Scholar]
  13. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  14. Lee H.-G., Kim S.-G., Im W.-T., Oh H.-M., Lee S.-T. 2009; Pedobacter composti sp. nov., isolated from compost. Int J Syst Evol Microbiol 59:345–349 [CrossRef][PubMed]
    [Google Scholar]
  15. Roh S. W., Quan Z.-X., Nam Y.-D., Chang H.-W., Kim K.-H., Kim M.-K., Im W.-T., Jin L., Kim S.-H. et al. other authors 2008; Pedobacter agri sp. nov., from soil. Int J Syst Evol Microbiol 58:1640–1643 [CrossRef][PubMed]
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48:165–177 [CrossRef][PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  20. Ten L. N., Liu Q.-M., Im W.-T., Lee M., Yang D.-C., Lee S.-T. 2006; Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 56:2565–2570 [CrossRef][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef][PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon J.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2006; Pedobacter sandarakinus sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1273–1277 [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon M.-H., Ten L. N., Im W.-T., Lee S.-T. 2007a; Pedobacter panaciterrae sp. nov., isolated from soil in South Korea. Int J Syst Evol Microbiol 57:381–386 [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kang S.-J., Oh H. W., Oh T.-K. 2007b; Pedobacter insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:1999–2003 [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Kang S.-J., Park S., Oh T.-K. 2007c; Pedobacter lentus sp. nov. and Pedobacter terricola sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:2089–2095 [CrossRef][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Oh T.-K. 2007d; Pedobacter terrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:2462–2466 [CrossRef][PubMed]
    [Google Scholar]
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