1887

Abstract

A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9, was isolated from soil collected on the Korean peninsula. Strain BR-9 grew optimally at pH 6.0–7.0, at 30 °C and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BR-9 belonged to the genus and clustered with DS-139 and WPCB189. Strain BR-9 exhibited 98.2 and 97.5 % 16S rRNA gene sequence similarity with DS-139 and WPCB189, respectively, and <96.7 % sequence similarity with the type strains of other species in the genus . Strain BR-9 contained MK-7 as the predominant menaquinone and iso-C and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acids. The DNA G+C content of strain BR-9 was 38.5 mol%. DNA–DNA relatedness between strain BR-9 and DS-139 and KCTC 12536 was 3.4–4.2 %, which indicated that the isolate was genetically distinct from these type strains. Strain BR-9 was also distinguishable by differences in phenotypic properties. On the basis of the data presented, strain BR-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BR-9 ( = KCTC 23344  = CCUG 60024).

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2012-01-01
2019-10-16
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References

  1. An D.-S. , Kim S.-G. , Ten L. N. , Cho C.-H. . ( 2009; ). Pedobacter daechungensis sp. nov., from freshwater lake sediment in South Korea. . Int J Syst Evol Microbiol 59:, 69–72. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baik K. S. , Park Y.-D. , Kim M. S. , Park S. C. , Moon E. Y. , Rhee M. S. , Choi J. H. , Seong C. N. . ( 2007; ). Pedobacter koreensis sp. nov., isolated from fresh water. . Int J Syst Evol Microbiol 57:, 2079–2083. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  6. Euzéby J. P. . ( 1997; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592.[CrossRef]
    [Google Scholar]
  7. Euzéby J. P. . ( 2010; ). List of prokaryotic names with standing in nomenclature. Last full update 5 August 2010. http://www.bacterio.cict.fr/p/pedobacter.html
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  9. Hwang C. Y. , Choi D. H. , Cho B. C. . ( 2006; ). Pedobacter roseus sp. nov., isolated from a hypertrophic pond, and emended description of the genus Pedobacter . . Int J Syst Evol Microbiol 56:, 1831–1836. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jeon Y. , Kim J. M. , Park J. H. , Lee S. H. , Seong C.-N. , Lee S.-S. , Jeon C. O. . ( 2009; ). Pedobacter oryzae sp. nov., isolated from rice paddy soil. . Int J Syst Evol Microbiol 59:, 2491–2495. [CrossRef] [PubMed]
    [Google Scholar]
  11. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  12. Kwon S.-W. , Kim B.-Y. , Lee K.-H. , Jang K.-Y. , Seok S.-J. , Kwon J.-S. , Kim W.-G. , Weon H.-Y. . ( 2007; ). Pedobacter suwonensis sp. nov., isolated from the rhizosphere of Chinese cabbage (Brassica campestris). . Int J Syst Evol Microbiol 57:, 480–484. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  14. Lee H.-G. , Kim S.-G. , Im W.-T. , Oh H.-M. , Lee S.-T. . ( 2009; ). Pedobacter composti sp. nov., isolated from compost. . Int J Syst Evol Microbiol 59:, 345–349. [CrossRef] [PubMed]
    [Google Scholar]
  15. Roh S. W. , Quan Z.-X. , Nam Y.-D. , Chang H.-W. , Kim K.-H. , Kim M.-K. , Im W.-T. , Jin L. , Kim S.-H. et al. & other authors ( 2008; ). Pedobacter agri sp. nov., from soil. . Int J Syst Evol Microbiol 58:, 1640–1643. [CrossRef] [PubMed]
    [Google Scholar]
  16. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  17. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  18. Steyn P. L. , Segers P. , Vancanneyt M. , Sandra P. , Kersters K. , Joubert J. J. . ( 1998; ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48:, 165–177. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  20. Ten L. N. , Liu Q.-M. , Im W.-T. , Lee M. , Yang D.-C. , Lee S.-T. . ( 2006; ). Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 56:, 2565–2570. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  22. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  23. Yoon J.-H. , Lee S. T. , Park Y.-H. . ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  24. Yoon J.-H. , Kang K. H. , Park Y.-H. . ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yoon J.-H. , Lee M.-H. , Kang S.-J. , Park S.-Y. , Oh T.-K. . ( 2006; ). Pedobacter sandarakinus sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 1273–1277. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon M.-H. , Ten L. N. , Im W.-T. , Lee S.-T. . ( 2007a; ). Pedobacter panaciterrae sp. nov., isolated from soil in South Korea. . Int J Syst Evol Microbiol 57:, 381–386. [CrossRef] [PubMed]
    [Google Scholar]
  27. Yoon J.-H. , Kang S.-J. , Oh H. W. , Oh T.-K. . ( 2007b; ). Pedobacter insulae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:, 1999–2003. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon J.-H. , Kang S.-J. , Park S. , Oh T.-K. . ( 2007c; ). Pedobacter lentus sp. nov. and Pedobacter terricola sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:, 2089–2095. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoon J.-H. , Kang S.-J. , Oh T.-K. . ( 2007; d). Pedobacter terrae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:, 2462–2466. [CrossRef] [PubMed]
    [Google Scholar]
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