1887

Abstract

A novel isolate from raw milk and four strains from diverse origins that were identified previously as , and showed a high degree of similarity in amplified rDNA restriction analysis, SDS-PAGE and routine phenotypic tests, whilst 16S rDNA sequence comparisons and DNA relatedness data showed that this taxon was different from related species. On the basis of these data, sp. nov. is proposed, with the type strain LMG 17892 (=DSM 15595=Logan B2188=MB 800).

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2004-03-01
2019-10-16
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References

  1. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Fahmy, F., Flossdorf, J. & Claus, D. ( 1985; ). The DNA base composition of the type strains of the genus Bacillus. Syst Appl Microbiol 6, 60–65.[CrossRef]
    [Google Scholar]
  4. Fritze, D., Flossdorf, J. & Claus, D. ( 1990; ). Taxonomy of alkaliphilic Bacillus strains. Int J Syst Bacteriol 40, 92–97.[CrossRef]
    [Google Scholar]
  5. Gibson, T. ( 1935; ). The urea-decomposing microflora of soils. I. Description and classification of the organisms. Zentbl Bakteriol Parasitenkd Infektkrankh Hyg Abt II 92, 364–380.
    [Google Scholar]
  6. Gordon, R. E. & Hyde, J. L. ( 1982; ). The Bacillus firmusBacillus lentus complex and pH 7·0 variants of some alkalophilic strains. J Gen Microbiol 128, 1109–1116.
    [Google Scholar]
  7. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N. ( 1973; ). The genus Bacillus (Agriculture Handbook no. 427). Washington, DC: Agricultural Research Service, United States Department of Agriculture.
  8. Gordon, R. E., Hyde, J. L. & Moore, J. A., Jr ( 1977; ). Bacillus firmus-Bacillus lentus: a series or one species? Int J Syst Bacteriol 27, 256–262.[CrossRef]
    [Google Scholar]
  9. Heyndrickx, M. & Scheldeman, P. ( 2002; ). Bacilli associated with spoilage in dairy products and other food. In Applications and Systematics of Bacillus and Relatives, pp. 65–82. Edited by R. C. W. Berkeley, M. Heyndrickx, N. A. Logan & P. De Vos. Oxford: Blackwell Science.
  10. Heyndrickx, M., Vauterin, L., Vandamme, P., Kersters, K. & De Vos, P. ( 1996; ). Applicability of combined amplified ribosomal DNA restriction analysis (ARDRA) patterns in bacterial phylogeny and taxonomy. J Microbiol Methods 26, 247–259.[CrossRef]
    [Google Scholar]
  11. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  12. Logan, N. A. & Berkeley, R. C. W. ( 1981; ). Classification and identification of members of the genus Bacillus using API tests. In The Aerobic Endospore-Forming Bacteria: Classification and Identification, pp. 105–140. Edited by R. C. W. Berkeley & M. Goodfellow. London: Academic Press.
  13. Logan, N. A. & Berkeley, R. C. W. ( 1984; ). Identification of Bacillus strains using the API system. J Gen Microbiol 130, 1871–1882.
    [Google Scholar]
  14. Logan, N. A., Lebbe, L., Hoste, B. & 7 other authors ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  15. Logan, N. A., Forsyth, G., Lebbe, L. & 8 other authors ( 2002; ). Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov. Int J Syst Evol Microbiol 52, 953–966.[CrossRef]
    [Google Scholar]
  16. Logan, N. A., Lebbe, L., Verhelst, A., Goris, J., Forsyth, G., Rodríguez-Díaz, M., Heyndrickx, M. & De Vos, P. ( 2004; ). Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54, 373–376.[CrossRef]
    [Google Scholar]
  17. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Bacterial Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester, UK: Wiley.
  18. Priest, F. G., Goodfellow, M. & Todd, C. ( 1988; ). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882.
    [Google Scholar]
  19. Scheldeman, P., Herman, L., Goris, J., De Vos, P. & Heyndrickx, M. ( 2002; ). Polymerase chain reaction identification of Bacillus sporothermodurans from dairy sources. J Appl Microbiol 92, 983–991.[CrossRef]
    [Google Scholar]
  20. Seki, T., Minoda, M., Yagi, J. & Oshima, Y. ( 1983; ). Deoxyribonucleic acid reassocation between strains of Bacillus firmus, Bacillus lentus, and intermediate strains. Int J Syst Bacteriol 33, 401–403.[CrossRef]
    [Google Scholar]
  21. Skerman, V. B. D., McGowan, V. & Sneath, P. H. A. ( 1980; ). Approved lists of bacterial names. Int J Syst Bacteriol 30, 225–420.[CrossRef]
    [Google Scholar]
  22. Vauterin, L. & Vauterin, P. ( 1992; ). Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur Microbiol 1, 37–41.
    [Google Scholar]
  23. Vauterin, L., Yang, P., Hoste, B., Vancanneyt, M., Civerolo, E. L., Swings, J. & Kersters, K. ( 1991; ). Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, fatty acid analysis, and DNA-DNA hybridization. Int J Syst Bacteriol 41, 535–542.[CrossRef]
    [Google Scholar]
  24. Werner, W. ( 1933; ). Botanische Beschreibung häufinger am Buttersäureabbau beteiligter sporenbildender Bakterienspezies. Zentbl Bakteriol Parasitenkd Infektkrankh Hyg Abt II 87, 446–475 (in German).
    [Google Scholar]
  25. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
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vol. , part 2, pp. 617 – 621

Neighbour-joining clustering of 16S rDNA sequences, showing the position of LMG 17892 .

Normalized computer profiles from SDS-PAGE analyses of whole-cell proteins.

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