1887

Abstract

Two strains of aerobic methanotrophic bacteria, AR4 and SOP9, were isolated from acidic (pH 3.8–4.0) peat bogs in Russia. Another phenotypically similar isolate, strain LAY, was obtained from an acidic (pH 4.0) forest soil in Germany. Cells of these strains were Gram-negative, non-pigmented, non-motile, thin rods that multiplied by irregular cell division and formed rosettes or amorphous cell conglomerates. Similar to species, strains AR4, SOP9 and LAY possessed only a soluble form of methane monooxygenase (sMMO) and lacked intracytoplasmic membranes. Growth occurred only on methane and methanol; the latter was the preferred growth substrate. mRNA transcripts of sMMO were detectable in cells when either methane or both methane and methanol were available. Carbon was assimilated via the serine and ribulose-bisphosphate (RuBP) pathways; nitrogen was fixed via an oxygen-sensitive nitrogenase. Strains AR4, SOP9 and LAY were moderately acidophilic, mesophilic organisms capable of growth between pH 3.5 and 7.2 (optimum pH 4.8–5.2) and at 4–33 °C (optimum 20–23 °C). The major cellular fatty acid was 18 : 1ω7 and the quinone was Q-10. The DNA G+C content was 55.6–57.5 mol%. The isolates belonged to the family of the class and were most closely related to the sMMO-possessing methanotrophs of the genus (96.4–97.0 % 16S rRNA gene sequence similarity), particulate MMO (pMMO)-possessing methanotrophs of the genus (96.1–97.0 %), facultative methylotrophs of the genus (96.1–96.3 %) and non-methanotrophic organotrophs of the genus (96.5–97.0 %). Phenotypically, strains AR4, SOP9 and LAY were most similar to species, but differed from members of this genus by cell morphology, greater tolerance of low pH, detectable activities of RuBP pathway enzymes and inability to grow on multicarbon compounds. Therefore, we propose a novel genus and species, gen. nov., sp. nov., to accommodate strains AR4, SOP9 and LAY. Strain AR4 ( = DSM 22108  = LMG 25277  = VKM B-2543) is the type strain of .

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2011-10-01
2019-10-15
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vol. , part 10, pp. 2456 - 2463

Unrooted neighbour-joining tree reconstructed on the basis of 170 deduced amino acid sites of partial gene sequences, showing the position of strain AR4 relative to acidic soil clones (ASC) retrieved in stable isotope probing-based studies and other proteobacterial methylotrophs.

Detection of and mRNA transcripts after growth of strain AR4 on methane or methanol or in the presence of both substrates.

Influence of medium pH and temperature on growth of strain AR4 .

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