1887

Abstract

A novel species, , is proposed for three methanotrophic strains (T4, TCh1 and TY1) isolated from acidic tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4, TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·5–6·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 17. The DNA G+C content of strain T4 is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of , (97 % similarity) and (97·5 % similarity). DNA–DNA hybridization values of strain T4 with K and BL2 were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name sp. nov. is proposed. Strain T4 (=DSM 15673=NCIMB 13949) is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02805-0
2004-01-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/1/ijs540151.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02805-0&mimeType=html&fmt=ahah

References

  1. Auman, A. J., Stolyar, S., Costello, A. M. & Lidstrom, M. E. ( 2000; ). Molecular characterization of methanotrophic isolates from freshwater lake sediment. Appl Environ Microbiol 66, 5259–5266.[CrossRef]
    [Google Scholar]
  2. Berestovskaya, Y. Y., Vasil'eva, L. V., Chestnykh, O. V. & Zavarzin, G. A. ( 2002; ). Methanotrophs of the psychrophilic microbial community of Russian arctic tundra. Microbiology (English translation of Mikrobiologiya) 71, 538–544.
    [Google Scholar]
  3. Costello, A. M. & Lidstrom, M. E. ( 1999; ). Molecular characterization of functional and phylogenetic genes from natural populations of methanotrophs in lake sediments. Appl Environ Microbiol 65, 5066–5074.
    [Google Scholar]
  4. Dedysh, S. N., Panikov, N. S. & Tiedje, J. M. ( 1998a; ). Acidophilic methanotrophic communities from Sphagnum peat bogs. Appl Environ Microbiol 64, 922–929.
    [Google Scholar]
  5. Dedysh, S. N., Panikov, N. S., Liesack, W., Großkopf, R., Zhou, J. & Tiedje, J. M. ( 1998b; ). Isolation of acidophilic methane-oxidizing bacteria from northern peat wetlands. Science 282, 281–284.[CrossRef]
    [Google Scholar]
  6. Dedysh, S. N., Liesack, W., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Semrau, J. D., Bares, A. M., Panikov, N. S. & Tiedje, J. M. ( 2000; ). Methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs, representing a novel subtype of serine-pathway methanotrophs. Int J Syst Evol Microbiol 50, 955–969.[CrossRef]
    [Google Scholar]
  7. Dedysh, S. N., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Semrau, J. D., Liesack, W. & Tiedje, J. M. ( 2002; ). Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog. Int J Syst Evol Microbiol 52, 251–261.
    [Google Scholar]
  8. Dunfield, P. F., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A. & Dedysh, S. N. ( 2003; ). Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol. Int J Syst Evol Microbiol 53, 1231–1239.[CrossRef]
    [Google Scholar]
  9. Holmes, A. J., Costello, A., Lidstrom, M. E. & Murrell, J. C. ( 1995; ). Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related. FEMS Microbiol Lett 132, 203–208.[CrossRef]
    [Google Scholar]
  10. Luft, J. H. ( 1964; ). Electron microscopy of cell extraneous coats as revealed by ruthenium red staining. J Cell Biol 23, 54A–55A.
    [Google Scholar]
  11. Lysenko, A. M., Gal'chenko, V. F. & Chernykh, N. A. ( 1988; ). Taxonomic study of obligate methanotrophic bacteria using the DNA–DNA hybridization technique. Microbiology (English translation of Mikrobiologiya) 57, 653–658.
    [Google Scholar]
  12. McDonald, I. R. & Murrell, J. C. ( 1997; ). The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. Appl Environ Microbiol 63, 3218–3224.
    [Google Scholar]
  13. Owen, R. J., Hill, L. R. & Lapage, S. P. ( 1969; ). Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7, 503–516.[CrossRef]
    [Google Scholar]
  14. Strunk, O. & Ludwig, W. ( 1996; ). arb: a Software Environment for Sequence Data. Munich, Germany: Technische Universität München. http://www.arb-home.de.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02805-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02805-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error