1887

Abstract

A novel species, , is proposed for three methanotrophic strains (T4, TCh1 and TY1) isolated from acidic tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4, TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·5–6·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 17. The DNA G+C content of strain T4 is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of , (97 % similarity) and (97·5 % similarity). DNA–DNA hybridization values of strain T4 with K and BL2 were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name sp. nov. is proposed. Strain T4 (=DSM 15673=NCIMB 13949) is the type strain.

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/content/journal/ijsem/10.1099/ijs.0.02805-0
2004-01-01
2024-04-26
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