1887

Abstract

Phenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family . 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family with <96 % sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including , and genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as gen. nov., sp. nov. (the type species) and genomospecies 1. The G+C content of the DNA was 39.5 mol%. The type strain of gen. nov., sp. nov. is M327/99/2 ( = CCUG 43067 = NCTC 13475 = 98-D-690B) and the reference strain of genomospecies 1 is M1765/96/5 ( = CCUG 59551 = NCTC 13474).

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2011-12-01
2019-10-14
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References

  1. Angen Ø. , Mutters R. , Caugant D. A. , Olsen J. E. , Bisgaard M. . ( 1999; ). Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA–DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.. Int J Syst Bacteriol 49:, 67–86. [CrossRef] [PubMed]
    [Google Scholar]
  2. Angen Ø. , Ahrens P. , Kuhnert P. , Christensen H. , Mutters R. . ( 2003; ). Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedisHaemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’. . Int J Syst Evol Microbiol 53:, 1449–1456. [CrossRef] [PubMed]
    [Google Scholar]
  3. Baker J. R. , Ross H. M. . ( 1992; ). The role of bacteria in phocine distemper. . Sci Total Environ 115:, 9–14. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bisgaard M. . ( 1993; ). Ecology and significance of Pasteurellaceae in animals. . Zentralbl Bakteriol 279:, 7–26.[PubMed] [CrossRef]
    [Google Scholar]
  5. Bisgaard M. , Christensen J. P. , Bojesen A. M. , Christensen H. . ( 2007; ). Avibacterium endocarditidis sp. nov., isolated from valvular endocarditis in chickens. . Int J Syst Evol Microbiol 57:, 1729–1734. [CrossRef] [PubMed]
    [Google Scholar]
  6. Bisgaard M. , Korczak B. M. , Busse H. J. , Kuhnert P. , Bojesen A. M. , Christensen H. . ( 2009; ). Classification of the taxon 2 and taxon 3 complex of Bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp. nov., Gallibacterium trehalosifermentans sp. nov. and Gallibacterium salpingitidis sp. nov.. Int J Syst Evol Microbiol 59:, 735–744. [CrossRef] [PubMed]
    [Google Scholar]
  7. Blackall P. J. , Christensen H. , Beckenham T. , Blackall L. L. , Bisgaard M. . ( 2005; ). Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov.. Int J Syst Evol Microbiol 55:, 353–362. [CrossRef] [PubMed]
    [Google Scholar]
  8. Blackall P. J. , Bojesen A. M. , Christensen H. , Bisgaard M. . ( 2007; ). Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov.. Int J Syst Evol Microbiol 57:, 666–674. [CrossRef] [PubMed]
    [Google Scholar]
  9. Christensen H. , Bisgaard M. . ( 2004; ). Revised definition of Actinobacillus sensu stricto isolated from animals. A review with special emphasis on diagnosis. . Vet Microbiol 99:, 13–30. [CrossRef] [PubMed]
    [Google Scholar]
  10. Christensen H. , Bisgaard M. . ( 2008; ). Taxonomy and biodiversity of members of Pasteurellaceae . . In Pasteurellaceae: Biology, Genomics and Molecular Aspects, pp. 1–26. Edited by Kuhnert P. , Christensen H. . . Norwich, UK:: Caister Academic Press;.
    [Google Scholar]
  11. Christensen H. , Bisgaard M. , Bojesen A. M. , Mutters R. , Olsen J. E. . ( 2003; ). Genetic relationships among avian isolates classified as Pasteurella haemolytica, ‘Actinobacillus salpingitidis’ or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov.. Int J Syst Evol Microbiol 53:, 275–287. [CrossRef] [PubMed]
    [Google Scholar]
  12. Christensen H. , Bisgaard M. , Aalbaek B. , Olsen J. E. . ( 2004; ). Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae . . Int J Syst Evol Microbiol 54:, 813–818. [CrossRef] [PubMed]
    [Google Scholar]
  13. Foster G. , Ross H. M. , Malnick H. , Willems A. , Garcia P. , Reid R. J. , Collins M. D. . ( 1996; ). Actinobacillus delphinicola sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from sea mammals. . Int J Syst Bacteriol 46:, 648–652. [CrossRef] [PubMed]
    [Google Scholar]
  14. Foster G. , Ross H. M. , Patterson I. A. P. , Hutson R. A. , Collins M. D. . ( 1998; ). Actinobacillus scotiae sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from porpoises (Phocoena phocoena). . Int J Syst Bacteriol 48:, 929–933. [CrossRef] [PubMed]
    [Google Scholar]
  15. Foster G. , Ross H. M. , Malnick H. , Willems A. , Hutson R. A. , Reid R. J. , Collins M. D. . ( 2000; ). Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). . Int J Syst Evol Microbiol 50:, 135–139. [CrossRef] [PubMed]
    [Google Scholar]
  16. Frederiksen W. . ( 1993; ). Ecology and significance of Pasteurellaceae in man – an update. . Zentralbl Bakteriol 279:, 27–34.[PubMed] [CrossRef]
    [Google Scholar]
  17. Garvie E. I. . ( 1978; ). Streptococcus raffinolactis Orla-Jensen and Hansen, a group N streptococcus found in raw milk. . Int J Syst Bacteriol 28:, 190–193. [CrossRef]
    [Google Scholar]
  18. Gregersen R. H. , Neubauer C. , Christensen H. , Bojesen A. M. , Hess M. , Bisgaard M. . ( 2009; ). Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae . . Int J Syst Evol Microbiol 59:, 1583–1588.[PubMed] [CrossRef]
    [Google Scholar]
  19. Korczak B. , Christensen H. , Emler S. , Frey J. , Kuhnert P. . ( 2004; ). Phylogeny of the family Pasteurellaceae based on rpoB sequences. . Int J Syst Evol Microbiol 54:, 1393–1399. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kuhnert P. , Korczak B. M. . ( 2006; ). Prediction of whole-genome DNA-DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA). . Microbiology 152:, 2537–2548. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kuhnert P. , Frey J. , Lang N. P. , Mayfield L. . ( 2002; ). Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. . Int J Syst Evol Microbiol 52:, 1391–1395. [CrossRef] [PubMed]
    [Google Scholar]
  22. Kuhnert P. , Korczak B. , Falsen E. , Straub R. , Hoops A. , Boerlin P. , Frey J. , Mutters R. . ( 2004; ). Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. . J Clin Microbiol 42:, 5542–5548. [CrossRef] [PubMed]
    [Google Scholar]
  23. Kuhnert P. , Korczak B. M. , Christensen H. , Bisgaard M. . ( 2007; ). Emended description of Actinobacillus capsulatus Arseculeratne 1962, 38AL . . Int J Syst Evol Microbiol 57:, 625–632. [CrossRef] [PubMed]
    [Google Scholar]
  24. Kuhnert P. , Scholten E. , Haefner S. , Mayor D. , Frey J. . ( 2010; ). Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen. . Int J Syst Evol Microbiol 60:, 44–50. [CrossRef] [PubMed]
    [Google Scholar]
  25. Kuiken T. , Hartmann M. G. . ( 1991; ). Cetacean pathology: dissection techniques and tissue sampling. . ECS Newsletter No. 17: (special issue).
    [Google Scholar]
  26. Mayor D. , Korczak B. M. , Christensen H. , Bisgaard M. , Frey J. , Kuhnert P. . ( 2006; ). Distribution of RTX toxin genes in strains of [Actinobacillus] rossii and [Pasteurella] mairii . . Vet Microbiol 116:, 194–201. [CrossRef] [PubMed]
    [Google Scholar]
  27. Mutters R. , Ihm P. , Pohl S. , Frederiksen W. , Mannheim W. . ( 1985; ). Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis and Pasteurella langaa . . Int J Syst Bacteriol 35:, 309–322. [CrossRef]
    [Google Scholar]
  28. Nørskov-Lauritsen N. , Kilian M. . ( 2006; ). Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. . Int J Syst Evol Microbiol 56:, 2135–2146. [CrossRef] [PubMed]
    [Google Scholar]
  29. Nørskov-Lauritsen N. , Bruun B. , Kilian M. . ( 2005; ). Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.. Int J Syst Evol Microbiol 55:, 449–456. [CrossRef] [PubMed]
    [Google Scholar]
  30. Osawa R. , Rainey F. , Fujisawa T. , Lang E. , Busse H. J. , Walsh T. P. , Stackebrandt E. . ( 1995; ). Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium. . Syst Appl Microbiol 18:, 368–373.[CrossRef]
    [Google Scholar]
  31. Patel S. M. , Mo J. H. , Walker M. T. , Adley B. , Noskin G. A. . ( 2004; ). Epidural abscess and osteomyelitis due to Actinobacillus actinomycetemcomitans . . Diagn Microbiol Infect Dis 50:, 283–285. [CrossRef] [PubMed]
    [Google Scholar]
  32. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
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vol. , part 12, pp. 3016 - 3022

Phylogenetic trees based on the 16S rRNA, partial , partial RNA and partial gene sequences.

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