1887

Abstract

A collection of 75 strains of (including all biovars and pathovars) and the type strains of (CFBP 4178) and (CFBP 3613) were studied by DNA–DNA hybridization, numerical taxonomy of 121 phenotypic characteristics, serology and 16S rRNA gene-based phylogenetic analyses. From analysis of 16S rRNA gene sequences, it was deduced that strains and CFBP 4178 formed a clade distinct from the genera and ; therefore, it is proposed to transfer all the strains to a novel genus, gen. nov. By DNA–DNA hybridization, the strains of were distributed among six genomic species: genomospecies 1 harbouring 16 strains of biovar 3 and four strains of biovar 8, genomospecies 2 harbouring 16 strains of biovar 3, genomospecies 3 harbouring two strains of biovar 6 and five strains of biovar 5, genomospecies 4 harbouring five strains of biovar 2, genomospecies 5 harbouring six strains of biovar 1, four strains of biovar 7 and five strains of biovar 9 and genomospecies 6 harbouring five strains of biovar 4 and CFBP 4178. Two strains of biovar 3 remained unclustered. Biochemical criteria, deduced from a numerical taxonomic study of phenotypic characteristics, and serological reactions allowed discrimination of the strains belonging to the six genomic species. Thus, it is proposed that the strains clustered in these six genomic species be assigned to the species sp. nov. (type strain CFBP 2052=NCPPB 2538), sp. nov. (type strain CFBP 1269=NCPPB 898), comb. nov. (subdivided into two biovars, bv. and bv. ), sp. nov. (type strain CFBP 2051=NCPPB 2976), sp. nov. (type strain CFBP 1200=NCPPB 453) and comb. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02791-0
2005-07-01
2019-09-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551415.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02791-0&mimeType=html&fmt=ahah

References

  1. Avrova, A. O., Hyman, L. H., Toth, R. L. & Toth, I. K. ( 2002; ). Application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria Erwinia carotovora and Erwinia chrysanthemi. Appl Environ Microbiol 68, 1499–1508.[CrossRef]
    [Google Scholar]
  2. Boccara, M., Vedel, R., Lalo, D., Lebrun, M. H. & Lafay, J. F. ( 1991; ). Genetic diversity and host range in strains of Erwinia chrysanthemi. Mol Plant Microb Interact 4, 293–299.[CrossRef]
    [Google Scholar]
  3. Brenner, D. J., Steigerwalt, A. G., Miklos, G. V. & Fanning, G. R. ( 1973; ). Deoxyribonucleic acid relatedness among erwiniae and other Enterobacteriaceae: the soft-rot organisms (genus Pectobacterium Waldee). Int J Syst Bacteriol 23, 205–216.[CrossRef]
    [Google Scholar]
  4. Brenner, D. J., Fanning, G. R. & Steigerwalt, A. G. ( 1977; ). Deoxyribonucleic acid relatedness among erwiniae and other enterobacteria. II. Corn stalk rot bacterium and Pectobacterium chrysanthemi. Int J Syst Bacteriol 27, 211–221.[CrossRef]
    [Google Scholar]
  5. Brenner, D. J., McWhorter, A. C., Knutson, J. K. & Steigerwalt, A. G. ( 1982; ). Escherichia vulneris: a new species of Enterobacteriacae associated with human wounds. J Clin Microbiol 15, 1133–1140.
    [Google Scholar]
  6. Burkholder, W. H. ( 1957; ). Genus VI. Erwinia Winslow et al. 1917. In Bergey's Manual of Determinative Bacteriology, 7th edn, pp. 349–359. Edited by R. S. Breed, E. G. D. Murray & N. R. Smith. Baltimore: Williams & Wilkins.
  7. Burkholder, W. H., MacFadden, L. H. & Dimock, A. H. ( 1953; ). A bacterial blight of chrysanthemums. Phytopathology 43, 522–525.
    [Google Scholar]
  8. CABI ( 2001; ). Crop Protection Compendium. Wallingford, UK: CAB International. CD-ROM; http://www.cabicompendium.org/cpc
  9. Campbell, W. A. ( 1947; ). A bacterial root and stem disease of gayule. Phytopathology 37, 271–277.
    [Google Scholar]
  10. Crosa, J. H., Brenner, D. J. & Falkow, S. ( 1973; ). Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol 115, 904–911.
    [Google Scholar]
  11. Descamp, P. & Véron, M. ( 1981; ). Une méthode de choix des caractères d'identification basée sur le théorème de Bayes et la mesure de l'information. Ann Microbiol 132B, 157–170 (in French).
    [Google Scholar]
  12. Dickey, R. S. ( 1979; ). Erwinia chrysanthemi: a comparative study of phenotypic properties of strains from several hosts and other Erwinia species. Phytopathology 69, 324–329.[CrossRef]
    [Google Scholar]
  13. Dickey, R. S. ( 1981; ). Erwinia chrysanthemi: reaction of eight plants to strains from several hosts and to strains of other Erwinia species. Phytopathology 71, 23–29.[CrossRef]
    [Google Scholar]
  14. Dickey, R. S. & Victoria, J. I. ( 1980; ). Taxonomy and emended description of strains of Erwinia isolated from Musa paradisiaca Linnaeus. Int J Syst Bacteriol 30, 129–134.[CrossRef]
    [Google Scholar]
  15. Dickey, R. S., Zumoff, C. H. & Uyemoto, J. K. ( 1984; ). Erwinia chrysanthemi: serological relationships among strains from several hosts. Phytopathology 74, 1388–1434.[CrossRef]
    [Google Scholar]
  16. Dickey, R. S., Claflin, L. E. & Zumoff, C. H. ( 1987; ). Erwinia chrysanthemi: serological comparisons of strains from Zea mays and other hosts. Phytopathology 77, 426–430.[CrossRef]
    [Google Scholar]
  17. Dye, D. W. ( 1969; ). A taxonomic study of the genus Erwinia. II. The ‘carotovora’ group. N Z J Sci 12, 81–97.
    [Google Scholar]
  18. Fernandez-Borrero, O. & Lopez-Duque, S. ( 1970; ). Pudricion acuosa des seudo tallo del plátano (Musa paradisiaca) causada por Erwinia paradisiaca, n. sp. CENICAFE 21, 3–44 (in Spanish).
    [Google Scholar]
  19. Felsenstein, J. ( 1995; ). phylip (phylogeny inference package), version 3.57c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  20. Gardan, L., Gouy, C., Christen, R. & Samson, R. ( 2003; ). Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. Int J Syst Evol Microbiol 53, 381–391.[CrossRef]
    [Google Scholar]
  21. Gascuel, O. ( 1997; ). bionj: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14, 685–695.[CrossRef]
    [Google Scholar]
  22. Graham, D. C. ( 1964; ). Taxonomy of the soft rot coliform bacteria. Annu Rev Phytopathol 2, 13–42.[CrossRef]
    [Google Scholar]
  23. Graham, D. C. ( 1972; ). Identification of soft rot coliform bacteria. In Proceedings of the 3rd International Conference on Plant-Pathogenic Bacteria, pp. 273–279. Edited by H. P. Maas-Geesteranus. Wageningen: Pudoc.
  24. Grimont, P. A. D., Popoff, M. Y., Grimont, F., Coynault, C. & Lemelin, M. ( 1980; ). Reproducibility and correlation study of three deoxynucleic acid hybridization procedures. Curr Microbiol 4, 325–330.[CrossRef]
    [Google Scholar]
  25. Guillorit-Rondeau, C., Malandrin, L. & Samson, R. ( 1996; ). Identification of two serological flagellar types (H1 and H2) in Pseudomonas syringae pathovars. Eur J Plant Pathol 102, 99–104.[CrossRef]
    [Google Scholar]
  26. Hauben, L., Moore, E. R. B., Vauterin, L., Steenackers, M., Mergaert, J., Verdonck, L. & Swings, J. ( 1998; ). Phylogenetic position of phytopathogens within the Enterobacteriaceae. Syst Appl Microbiol 21, 384–397.[CrossRef]
    [Google Scholar]
  27. Hellmers, E. ( 1955; ). Bacterial wilt of carnations. Gard Chron 137, 194.
    [Google Scholar]
  28. Hildebrand, D. C., Schroth, M. N. & Thomson, S. ( 1978; ). Nutritional properties useful for identification of soft-rotting Erwinia species. In Proceedings of the 4th International Conference on Plant-Pathogenic Bacteria, pp. 561–562. Edited by M. Ridé. Angers: INRA.
  29. Janse, J. D. & Ruissen, M. A. ( 1988; ). Characterization and classification of Erwinia chrysanthemi strains from several hosts in The Netherlands. Phytopathology 78, 800–808.[CrossRef]
    [Google Scholar]
  30. Lelliott, R. A. & Dickey, R. S. ( 1984; ). Genus VII. Erwinia Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1920, 209AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 469–476. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  31. Martinec, T. & Kocur, M. ( 1963; ). Taxonomicka studie rodu Erwinia. Fol Biol 2, 1–163 (in Czech).
    [Google Scholar]
  32. McFadden, L. A. ( 1961; ). Bacterial stem and leaf rot of Dieffenbachia in Florida. Phytopathology 51, 663–668.
    [Google Scholar]
  33. Nassar, A., Bertheau, Y., Dervin, C., Narcy, J. P. & Lemattre, M. ( 1994; ). Ribotyping of Erwinia chrysanthemi strains in relation to their pathogenic and geographic distribution. Appl Environ Microbiol 60, 3781–3789.
    [Google Scholar]
  34. Nassar, A., Darrasse, A., Lemattre, M., Kotoujansky, A., Dervin, C., Vedel, R. & Bertheau, Y. ( 1996; ). Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes. Appl Environ Microbiol 62, 2228–2235.
    [Google Scholar]
  35. Ngwira, N. & Samson, R. ( 1990; ). Erwinia chrysanthemi: description of two new biovars (bv 8 and bv 9) isolated from kalanchoe and maize host plants. Agronomie 10, 341–345.[CrossRef]
    [Google Scholar]
  36. Perrière, G. & Gouy, M. ( 1996; ). WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  37. Rasolofo, R. & Dadant, R. ( 1962; ). Dépérissement du géranium rosat Pelargonium capitatum aux Comores. Agron Trop 12, 1084–1088 (in French).
    [Google Scholar]
  38. Sabet, K. A. ( 1954; ). A new bacterial disease of maize in Egypt. Emp J Exp Agric 22, 65–67.
    [Google Scholar]
  39. Samson, R. ( 1973; ). Les erwinias pectinolytiques. II – Recherches sur les antigènes somatiques d'Erwinia carotovora var. chrysanthemi (Burkholder) Dye. Ann Phytopathol 5, 377–388 (in French).
    [Google Scholar]
  40. Samson, R. & Nassan-Agha, N. ( 1978; ). Biovars and serovars among strains of Erwinia chrysanthemi. In Proceedings of the 4th International Conference on Plant-Pathogenic Bacteria, pp. 547–553. Edited by M. Ridé. Angers: INRA.
  41. Samson, R., Poutier, F., Sailly, M. & Jouan, B. ( 1987; ). Caractérisation des Erwinia chrysanthemi isolées de Solanum tuberosum et d'autres plantes-hôtes selon les biovars et sérogroupes. Bull OEPP 17, 11–16 (in French).[CrossRef]
    [Google Scholar]
  42. Samson, R., Ngwira, N. & Rivera, N. ( 1990; ). Biochemical and serological diversity of Erwinia chrysanthemi. In Plant-Pathogenic Bacteria, Proceedings of the 7th International Conference on Plant-Pathogenic Bacteria, pp. 895–900. Edited by Z. Klement. Budapest: Akademia Kiado.
  43. Skerman, V. B. D., McGowan, V. & Sneath, P. H. A. ( 1980; ). Approved lists of bacterial names. Int J Syst Bacteriol 30, 225–420.[CrossRef]
    [Google Scholar]
  44. Sneath, P. H. A. & Sokal, R. R. ( 1973; ). Numerical Taxonomy: the Principles of Numerical Classification. San Francisco: W. H. Freeman.
  45. Sutra, L., Christen, R., Bollet, C., Simoneau, P. & Gardan, L. ( 2001; ). Samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of Erythrina sp., and discrimination of plant-pathogenic Enterobacteriaceae by phenotypic features. Int J Syst Evol Microbiol 51, 1291–1304.
    [Google Scholar]
  46. Thomson, S. V., Hildebrand, D. C. & Schroth, M. N. ( 1981; ). Identification and nutritional differentiation of the Erwinia sugar beet pathogen from members of Erwinia carotovora and Erwinia chrysanthemi. Phytopathology 71, 1037–1042.[CrossRef]
    [Google Scholar]
  47. Verdonck, L., Mergaert, J., Rijckaert, C., Swings, J., Kersters, K. & De Ley, J. ( 1987; ). Genus Erwinia: numerical analysis of phenotypic features. Int J Syst Bacteriol 37, 4–18.[CrossRef]
    [Google Scholar]
  48. Waldee, E. L. ( 1945; ). Comparative studies of some peritrichous phytopathogenic bacteria. Iowa State J Sci 19, 435–484.
    [Google Scholar]
  49. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  50. Yakrus, M. & Schaad, N. W. ( 1979; ). Serological relationships among strains of Erwinia chrysanthemi. Phytopathology 69, 517–522.[CrossRef]
    [Google Scholar]
  51. Young, J. M., Dye, D. W., Bradbury, J. F., Panagopoulos, C. G. & Robbs, C. F. ( 1978; ). A proposed nomenclature and classification for plant-pathogenic bacteria. N Z J Agric Res 21, 153–177.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02791-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02791-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error