1887

Abstract

A protease-producing marine bacterium, designated CF12-14, was isolated from sediment of the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain CF12-14 formed a separate lineage within the genus (). The isolate showed the highest 16S rRNA gene sequence similarity with ISL-52 (94.7 %), CL-SP19 (94.6 %) and other members of the genus (91.9–94.6 %). Cells were Gram-negative, aerobic, flagellated, straight or slightly curved, and often formed buds and prosthecae. Strain CF12-14 grew at 4–42 °C (optimum 30–35 °C) and with 0.1–15 % (w/v) NaCl (optimum 2–3 %). The isolate reduced nitrate to nitrite and hydrolysed DNA, but did not produce acids from sugars. The predominant cellular fatty acids were iso-C (27.4 %), iso-C (16.0 %) and iso-Cω9 (15.8 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone 8. The DNA G+C content was 50.4 mol%. The phylogenetic, phenotypic and chemotaxonomic data supported the conclusion that CF12-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF12-14 ( = CCTCC AB 208166 = KACC 13974).

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2012-02-01
2020-01-25
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References

  1. Bauer A. W. , Kirby W. M. M. , Sherris J. C. , Turck M. . ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  2. Brettar I. , Christen R. , Höfle M. G. . ( 2003; ). Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 53:, 407–413. [CrossRef] [PubMed]
    [Google Scholar]
  3. Buck J. D. . ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  4. Choi D. H. , Cho B. C. . ( 2005; ). Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. . Int J Syst Evol Microbiol 55:, 379–383. [CrossRef] [PubMed]
    [Google Scholar]
  5. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  6. Donachie S. P. , Hou S. , Gregory T. S. , Malahoff A. , Alam M. . ( 2003; ). Idiomarina loihiensis sp. nov., a halophilic γ-Proteobacterium from the Lō‘ihi submarine volcano, Hawai‘i. . Int J Syst Evol Microbiol 53:, 1873–1879. [CrossRef] [PubMed]
    [Google Scholar]
  7. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Hu Z.-Y. , Li Y. . ( 2007; ). Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. . Int J Syst Evol Microbiol 57:, 2572–2577. [CrossRef] [PubMed]
    [Google Scholar]
  9. Ivanova E. P. , Romanenko L. A. , Chun J. , Matte M. H. , Matte G. R. , Mikhailov V. V. , Svetashev V. I. , Huq A. , Maugel T. , Colwell R. R. . ( 2000; ). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov.. Int J Syst Evol Microbiol 50:, 901–907. [CrossRef] [PubMed]
    [Google Scholar]
  10. Ivanova E. P. , Flavier S. , Christen R. . ( 2004; ). Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov.. Int J Syst Evol Microbiol 54:, 1773–1788. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jean W. D. , Shieh W. Y. , Chiu H.-H. . ( 2006; ). Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . . Int J Syst Evol Microbiol 56:, 899–905. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jean W. D. , Leu T.-Y. , Lee C.-Y. , Chu T.-J. , Lin S. Y. , Shieh W. Y. . ( 2009; ). Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. . Int J Syst Evol Microbiol 59:, 53–59. [CrossRef] [PubMed]
    [Google Scholar]
  13. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  14. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  15. Kwon S.-W. , Kim B.-Y. , Weon H.-Y. , Baek Y.-K. , Koo B.-S. , Go S.-J. . ( 2006; ). Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. . Int J Syst Evol Microbiol 56:, 2229–2233. [CrossRef] [PubMed]
    [Google Scholar]
  16. Martínez-Cánovas M. J. , Béjar V. , Martínez-Checa F. , Páez R. , Quesada E. . ( 2004; ). Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. . Int J Syst Evol Microbiol 54:, 1793–1797. [CrossRef] [PubMed]
    [Google Scholar]
  17. Mesbah M. , Premachandran U. , Whitman W. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  18. Park S. C. , Lim C. H. , Baik K. S. , Lee K. H.-S. , Lee J.-S. , Seong C. N. . ( 2010; ). Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater. . Int J Syst Evol Microbiol 60:, 2071–2075. [CrossRef] [PubMed]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Taborda M. , Antunes A. , Tiago I. , Veríssimo A. , Nobre M. F. , da Costa M. S. . ( 2009; ). Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . . Syst Appl Microbiol 32:, 371–378. [CrossRef] [PubMed]
    [Google Scholar]
  22. Taborda M. , Antunes A. , Tiago I. , Veríssimo A. , Nobre M. F. , da Costa M. S. . ( 2010; ). Idiomarina donghaiensis comb. nov., Idiomarina insulisalsae sp. nov., Idiomarina marina comb. nov., Idiomarina maritima comb. nov., Idiomarina sediminum comb. nov., Idiomarina tainanensis comb. nov. and Idiomarina taiwanensis comb. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 131. . Int J Syst Evol Microbiol 60:, 1–2. [CrossRef]
    [Google Scholar]
  23. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wang L. , Lai Q. , Fu Y. , Chen H. , Wang W. , Wang J. , Sun F. , Shao Z. . ( 2011; ). Idiomarina xiamenensis sp. nov., isolated from surface seawater, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov.. Int J Syst Evol Microbiol 61:, 969–973.[CrossRef]
    [Google Scholar]
  25. Wu Y.-H. , Shen Y.-Q. , Xu X.-W. , Wang C.-S. , Oren A. , Wu M. . ( 2009; ). Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea. . Int J Syst Evol Microbiol 59:, 1321–1325. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon J.-H. , Jung S.-Y. , Jung Y.-T. , Oh T.-K. . ( 2007; ). Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. . Int J Syst Evol Microbiol 57:, 2503–2506. [CrossRef] [PubMed]
    [Google Scholar]
  27. Zhou M.-Y. , Chen X.-L. , Zhao H.-L. , Dang H.-Y. , Luan X.-W. , Zhang X.-Y. , He H.-L. , Zhou B.-C. , Zhang Y.-Z. . ( 2009; ). Diversity of both the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China sea. . Microb Ecol 58:, 582–590. [CrossRef] [PubMed]
    [Google Scholar]
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