1887

Abstract

An aerobic, non-motile actinobacterium, strain QAIII60, was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained -diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C, iso-C, iso-C H and Cω6. The isoprenoid quinones consisted of MK-9(H) and MK-10(H). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60 formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus . A number of physiological characteristics differentiated the isolate from members of the genus . On the basis of these data, we propose that strain QAIII60 ( = CGMCC 4.4663  = NBRC 106673) be assigned as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.027722-0
2011-12-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/12/2928.html?itemId=/content/journal/ijsem/10.1099/ijs.0.027722-0&mimeType=html&fmt=ahah

References

  1. Ara I. , Tsetseg B. , Daram D. , Suto M. , Ando K. . ( 2011; ). Actinophytocola burenkhanensis sp. nov., isolated from Mongolian soil. . Int J Syst Evol Microbiol 61:, 1033–1038. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  4. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  5. Embley T. M. , Smida J. , Stackebrandt E. . ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and the description of Pseudonocardiaceae fam. nov.. Syst Appl Microbiol 11:, 44–52.[CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  10. Indananda C. , Matsumoto A. , Inahashi Y. , Takahashi Y. , Duangmal K. , Thamchaipenet A. . ( 2010; ). Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 60:, 1141–1146. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kovács N. . ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703–704. [CrossRef] [PubMed]
    [Google Scholar]
  12. Labeda D. P. , Goodfellow M. , Chun J. , Zhi X. Y. , Li W. J. . ( 2011; ). Reassessment of the systematics of the suborder Pseudonocardineae: transfer of the genera within the family Actinosynnemataceae Labeda and Kroppenstedt 2000 emend. Zhi et al. 2009 into an emended family Pseudonocardiaceae Embley et al. 1989 emend. Zhi et al. 2009. . Int J Syst Evol Microbiol 61:, 1259–1264. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lechevalier M. P. , Lechevalier H. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  14. Lechevalier M. P. , De Bièvre C. , Lechevalier H. A. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  15. MacFaddin J. F. . ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , Hutchinson I. G. , Caldicott A. B. , Goodfellow M. . ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188:, 221–233. [CrossRef]
    [Google Scholar]
  18. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinies and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  19. Otoguro M. , Yamamura H. , Tamura T. , Irzaldi R. , Ratnakomala S. , Ridwan R. , Kartina G. , Triana E. , Nurkanto A. et al. & other authors ( 2011; ). Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61:, 834–838. [CrossRef] [PubMed]
    [Google Scholar]
  20. Qiu D. , Ruan J. , Huang Y. . ( 2008; ). Selective isolation and rapid identification of members of the genus Micromonospora . . Appl Environ Microbiol 74:, 5593–5597. [CrossRef] [PubMed]
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  23. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  24. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  25. Tamura T. , Hatano K. . ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:, 2119–2125. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. & other authors ( 1987; ). Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.027722-0
Loading
/content/journal/ijsem/10.1099/ijs.0.027722-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2928 - 2932

Cultural characteristics of sp. nov. QAIII60 and type strains of the genus .

Polar lipid profiles of sp. nov. QAIII60 and NBRC 105883 , separated by two-dimensional TLC and detected with molybdatophosphoric acid.

[PDF file of Supplementary Table and Figure](126 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error