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Abstract

Two mesophilic methanogenic strains, designated TS-2 and GH, were isolated from sediments of Tuosu lake and Gahai lake, respectively, in the Qaidam basin, Qinghai province, China. Cells of both isolates were rods (about 0.3–0.5×2–5 µm) with blunt rounded ends and Gram-staining-positive. Strain TS-2 was motile with one or two polar flagella and used only H/CO for growth and methanogenesis. Strain GH was non-motile, used both H/CO and formate and displayed a variable cell arrangement depending on the substrate: long chains when growing in formate (50 mM) or under high pressure H and single cells under low pressure H. Phylogenetic analysis based on 16S rRNA gene sequences placed the two isolates in the genus . Strain TS-2 was most closely related to NBRC 105226 (96 % 16S rRNA gene sequence similarity). Phylogenetic analysis based on the alpha subunit of methyl-coenzyme M reductase also supported the affiliation of the two isolates with the genus . DNA–DNA relatedness between the isolates and DSM 3387 was 39–53 %. Hence we propose two novel species, sp. nov. (type strain TS-2 = AS 1.5093 = JCM 15415) and sp. nov. (type strain GH = AS 1.5092 = JCM 15416).

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2011-12-01
2020-01-28
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vol. , part 12, pp. 2974 - 2978

Growth rates of sp. nov. TS-2 (open circles) and sp. nov. GH (solid circles) in H /CO medium with varying NaCl concentration, temperature and pH



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