1887

Abstract

A Gram-negative, aerobic, halophilic bacterium, designated strain JC2041, was isolated from a sediment sample of getbol, the Korean tidal flat. Results of 16S rDNA sequence analyses indicated a moderate relationship to within the - (94·9 % similarity). Depending on the tree-making algorithm used, the isolate either formed a monophyletic clade with or was recovered as a sister group of a class containing the genera and . Phenotypic features of the getbol isolate were similar to those of , but several physiological and chemotaxonomic properties, including nitrate reduction, amylase, lecithinase, Tweenase and utilization of 13 carbon sources, distinguished strain JC2041 from . The polyphasic data presented in this study indicate that the isolate should be classified as a novel species in the genus . The name sp. nov. is therefore proposed for the getbol isolate; the type strain is JC2041 (=IMSNU 14005=KCTC 12041=DSM 15355).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02748-0
2004-03-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/2/ijs540377.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02748-0&mimeType=html&fmt=ahah

References

  1. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D. ( 1972; ). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429.
    [Google Scholar]
  2. Bowman, J. P., Gosink, J. J., McCammon, S. A., Lewis, T. E., Nichols, D. S., Nichols, P. D., Skerratt, J. H., Staley, J. T. & McMeekin, T. A. ( 1998; ). Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6ω3). Int J Syst Bacteriol 48, 1171–1180.[CrossRef]
    [Google Scholar]
  3. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  4. Chun, J., Bae, K. S., Moon, E. Y., Jung, S.-O., Lee, H. K. & Kim, S.-J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  5. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  6. Deming, J. W., Somers, L. K., Straube, W. L., Swartz, D. G. & Macdonald, M. T. ( 1988; ). Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov. Syst Appl Microbiol 10, 152–160.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogenetic inference package) version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  9. Fitch, W. M. ( 1972; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.
    [Google Scholar]
  10. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  11. Ivanova, E. P., Romanenko, L. A., Chun, J. & 7 other authors ( 2000; ). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50, 901–907.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  14. Macián, M. C., Ludwig, W., Schleifer, K. H., Garay, E. & Pujalte, M. J. ( 2001; ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. Int J Syst Evol Microbiol 51, 1283–1289.
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., O’Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sawabe, T., Makino, H., Tatsumi, M., Nakano, K., Tajima, K., Iqbal, M. M., Yumoto, I., Ezura, Y. & Christen, R. ( 1998; ). Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica. Int J Syst Bacteriol 48, 769–774.[CrossRef]
    [Google Scholar]
  19. Shiba, T. ( 1992; ). The genus Roseobacter. In The Prokaryotes, 2nd edn, pp. 2156–2159. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  20. Swofford, D. L. ( 1998; ). Phylogenetic analysis using parsimony (PAUP), version 4. Sunderland, MA: Sinauer Associates.
  21. Yi, H. & Chun, J. ( 2002; ). Remarkable cultured bacterial biodiversity in getbol, the tidal flat of Korea. In Proceedings of the International Union of Microbiological Societies World Congresses, p. 165. Paris: IUMS.
  22. Yi, H., Chang, Y.-H., Oh, H. W., Bae, K. S. & Chun, J. ( 2003; ). Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. Int J Syst Evol Microbiol 53, 1013–1018.[CrossRef]
    [Google Scholar]
  23. Yumoto, I., Kawasaki, K., Iwata, H., Matsuyama, H. & Okuyama, H. ( 1998; ). Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium. Int J Syst Bacteriol 48, 1357–1362.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02748-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02748-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error