1887

Abstract

An actinobacterial strain was isolated from marine sediment taken from the Troitsa Bay of the Gulf of Peter the Great, East Siberian Sea, and subjected to a taxonomic investigation. The isolate, designated KMM 3905, was Gram-positive, aerobic, non-motile and coccoid. The strain tolerated up to 15 % NaCl in growth media, although its presence was not essential for growth. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus . The strain formed an independent phylogenetic line in the 16S rRNA gene tree encompassed by members of . Comparison of phenotypic characteristics also supported its assignment to a novel species within . The name sp. nov. (type strain KMM 3905=KCTC 9943) is proposed for this halotolerant actinobacterium from the marine environment.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02742-0
2004-09-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541617.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02742-0&mimeType=html&fmt=ahah

References

  1. Boháček, J., Kocur, M. & Martinec, T. ( 1969; ). Deoxyribonucleic acid base composition of Micrococcus roseus. Antonie van Leeuwenhoek 35, 185–188.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1993; ). phylip (Phylogenetic Inference Package), version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  4. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  5. Han, S. K., Nedashkovskaya, O. I., Mikhailov, V. V., Kim, S. B. & Bae, K. S. ( 2003; ). Salinibacterium amurskyense gen. nov., sp. nov., a novel genus of the family Microbacteriaceae from the marine environment. Int J Syst Evol Microbiol 53, 2061–2066.[CrossRef]
    [Google Scholar]
  6. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M. ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef]
    [Google Scholar]
  7. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  8. Kloos, W. E. ( 1969; ). Transformation of Micrococcus lysodeikticus by various members of the family Micrococcaceae. J Gen Microbiol 59, 247–255.[CrossRef]
    [Google Scholar]
  9. Kloos, W. E., Tornabene, T. G. & Schleifer, K. H. ( 1974; ). Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae. Int J Syst Bacteriol 24, 79–101.[CrossRef]
    [Google Scholar]
  10. Kocur, M. ( 1986; ). Genus Micrococcus Cohn 1872. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1004–1008. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  11. Kocur, M., Bergan, T. & Mortensen, N. ( 1971; ). DNA base composition of Gram-positive cocci. J Gen Microbiol 69, 167–183.[CrossRef]
    [Google Scholar]
  12. Kocur, M., Kloos, W. E. & Schleifer, K. H. ( 1991; ). The genus Micrococcus. In The Prokaryotes, 2nd edn, vol. 2, pp. 1300–1311. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  13. Kovács, G., Burghardt, J., Pradella, S., Schumann, P., Stackebrandt, E. & Màrialigeti, K. ( 1999; ). Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). Int J Syst Bacteriol 49, 167–173.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  15. Ogasawara-Fujita, N. & Sakaguchi, K. ( 1976; ). Classification of micrococci on the basis of deoxyribonucleic acid homology. J Gen Microbiol 94, 97–106.[CrossRef]
    [Google Scholar]
  16. Reddy, G. S. N., Prakash, J. S. S., Prabahar, V., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2003; ). Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53, 183–187.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02742-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02742-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error