1887

Abstract

A pigmented, slowly growing complex AccuProbe-positive organism was isolated from the sputum and pleural fluid of a 72-year-old female with bronchiectasis. The unusual morphology of the organism prompted further identification by 16S rRNA gene sequencing, revealing a perfect identity with previously uncharacterized strain sp. MCRO 8 (GenBank accession no. X93034), with the closest established species by 16S rDNA analysis being . HPLC of the organism corresponded to previously obtained patterns identified as -like and, upon sequence evaluation of a selection of strains with a similar profile, more were subsequently identified as MCRO 8. A total of 16 strains isolated from human respiratory samples were evaluated in the characterization of this novel species, for which the name sp. nov. is proposed. The type strain is strain 00-250 (=ATCC BAA-544=DSM 44616=CIP 108114).

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2004-05-01
2019-09-15
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Demographics of the study strains. [PDF](13 KB)

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Colony morphology of sp. nov. on Middelbrook 7H10 agar. Top: strain 01-244 (one of three strains obtained from the case study) at 3 weeks at 37 °C. Bottom: strain MB13237 (isolate from Québec) at 6 weeks at 37 °C.

IMAGE

Colony morphology of sp. nov. on Middelbrook 7H10 agar. Top: strain 01-244 (one of three strains obtained from the case study) at 3 weeks at 37 °C. Bottom: strain MB13237 (isolate from Québec) at 6 weeks at 37 °C.

IMAGE

Alignment of the ITS1 regions of sp. nov. and select mycobacteria. Nucleotides that differ from are shown. Dashes represent deletions. The length (in nt) of the ITS1 fragment from position 1 is indicated. Sequences were determined in our laboratory unless indicated by a GenBank accession number. [PDF](1911 KB)

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