1887

Abstract

Six ballistoconidium-forming yeast strains that were isolated from plant leaves collected on Changbai Mountain, north-east China, were assigned to the genus Ingold emend. Nakase & Boekhout due to the formation of asymmetrical ballistoconidia, cream-coloured colonies and Q-9 as the major ubiquinone. Two separate groups, representing two novel species, were recognized among these yeasts by 26S rDNA D1/D2 domain, internal transcribed spacer (ITS) region and 18S rDNA sequence analyses. The names sp. nov. (type strain, CB 346=AS 2.2310=CBS 9497) and sp. nov. (type strain, CB 286=AS 2.2303=CBS 9498) are proposed for these two species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02736-0
2003-11-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/6/ijs532085.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02736-0&mimeType=html&fmt=ahah

References

  1. Bai, F.-Y., Zhao, J.-H., Takashima, M., Jia, J.-H., Boekhout, T. & Nakase, T. ( 2002; ). Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 52, 2309–2314.[CrossRef]
    [Google Scholar]
  2. Boekhout, T. ( 1998; ). Diagnostic descriptions and key to presently accepted heterobasidiomycetous genera. In The Yeasts, a Taxonomic Study, 4th edn, pp. 627–634. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  3. Boekhout, T. & Nakase, T. ( 1998; ). Bensingtonia Ingold emend. Nakase & Boekhout. In The Yeasts, a Taxonomic Study, 4th edn, pp. 723–730. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  4. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fungsin, B., Hamamoto, M., Arunpairojana, V., Sukhumavasi, J., Atthasampunna, P. & Nakase, T. ( 2001; ). Bensingtonia thailandica sp. nov., a novel basidiomycetous yeast species isolated from plant leaves in Thailand. Int J Syst Evol Microbiol 51, 1209–1213.[CrossRef]
    [Google Scholar]
  7. Golubev, W. I. ( 1999; ). Mastigobasidium, a new teleomorphic genus for the perfect state of ballistosporous yeast Bensingtonia intermedia. Int J Syst Bacteriol 49, 1301–1305.[CrossRef]
    [Google Scholar]
  8. Hamamoto, M. & Nakase, T. ( 2000; ). Phylogenetic analysis of the ballistoconidium-forming yeast genus Sporobolomyces based on 18S rDNA sequences. Int J Syst Evol Microbiol 50, 1373–1380.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Makimura, K., Murayama, S. Y. & Yamaguchi, H. ( 1994; ). Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40, 358–364.[CrossRef]
    [Google Scholar]
  11. Nakase, T. & Boekhout, T. ( 1988; ). Emendation of the genus Bensingtonia Ingold. J Gen Appl Microbiol 34, 433–437.[CrossRef]
    [Google Scholar]
  12. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  13. Nakase, T. & Takashima, M. ( 1993; ). A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Rev 3, 33–34.
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Scorzetti, G., Fell, J. W., Fonseca, A. & Statzell-Tallman, A. ( 2002; ). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2, 495–517.[CrossRef]
    [Google Scholar]
  16. Sugita, T. & Nakase, T. ( 1999; ). Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida. Syst Appl Microbiol 22, 79–86.[CrossRef]
    [Google Scholar]
  17. Takashima, M., Suh, S.-O. & Nakase, T. ( 1995; ). Phylogenetic relationships among species of the genus Bensingtonia and related taxa based on the small subunit ribosomal DNA sequences. J Gen Appl Microbiol 41, 131–141.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  19. Yamada, Y. & Kondo, K. ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19, 59–77.[CrossRef]
    [Google Scholar]
  20. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02736-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02736-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 2085 – 2089

Phylogenetic trees drawn from neighbour-joining analysis of the (A) ITS region and (B) 18S rDNA sequences of the novel strains.

[Single PDF](136 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error