1887

Abstract

Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499 and CBS 10736) were identical and were closely related to , a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from by standard physiological tests. The other three isolates represent three novel species, closely related to . These three species could also be distinguished from other species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names sp. nov. (type strain CBS 8499  = NRRL Y-48408), sp. nov. (type strain CBS 10735  = NRRL Y-48410), sp. nov. (type strain CBS 8598  = NRRL Y-48407) and sp. nov. (type strain CBS 8594  = NRRL Y-48719) are proposed. and can also utilize limonene.

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2011-09-01
2019-12-11
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