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Abstract

During an investigation of bacterial diversity at hydrothermal vents on the Lō‘ihi Seamount, Hawai‘i, a novel bacterium (designated L2-TR) was cultivated, which shares 99·9 % 16S rRNA gene sequence similarity over 1415 nt with an uncultured eubacterium from sediment at a depth of 11 000 m in the Mariana Trench. The nearest cultivated neighbour of L2-TR, however, is KMM 227, with which it shares 98·9 % 16S rRNA sequence similarity. L2-TR differed from KMM 227 in several phenotypic respects, including growth at 46 °C and in medium that contained 20 % (w/v) NaCl. DNA–DNA hybridization data showed that L2-TR did not belong to the species (43·4 % DNA–DNA reassociation). Cells of L2-TR were Gram-negative rods, 0·35 μm wide and 0·7–1·0 μm long, which were occasionally up to 1·8 μm in length. Cells were motile by a single polar or subpolar flagellum. The major fatty acid in L2-TR was iso-C (32·6 %). The DNA G+C content was 47·4 mol%. Phenotypic and genotypic analyses indicated that L2-TR could be assigned to the genus but, based on significant phenotypic and genotypic differences, constituted a novel species within this genus, sp. nov., of which L2-TR (=ATCC BAA-735=DSM 15497) is the type strain.

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2003-11-01
2020-01-22
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vol. , part 6, pp. 1873 – 1879

Growth of sp. nov. in different NaCl concentrations.

Fatty acid composition of sp. nov. and of type strains of other members of the genus .

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