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Abstract

Two novel vibrio isolates (R-40492 and R-40493) originating from the zoanthid in Brazil in 2005 were taxonomically characterized by means of a polyphasic approach comprising multilocus sequence analysis (MLSA), DNA–DNA hybridization (DDH), Δ analysis and phenotypic characterization. Phylogenetic analysis based on 16S rRNA gene sequences showed that R-40492 and R-40493 fell within the genus and were most closely related to each other with 99 % similarity; similarities of these two novel isolates towards LMG 20536, LMG 20984, LMG 3896, LMG 25238 and LMG 20546 varied between 97.1 and 98.5 %. DDH experiments showed that the two isolates had less than 15 % relatedness to the phylogenetically most closely related species. R-40492 and R-40493 had 55–57 % relatedness to each other. The Δ between R-40492 and R-40493 was 6.12 °C. In addition, MLSA of concatenated sequences (16S rRNA, , , , , , and ; 6035 bp in length) showed that the two novel isolates formed a separate branch with less than 92 % concatenated gene sequence similarity towards known species of vibrios. Two novel species are proposed to accommodate these novel isolates, namely sp. nov. (type strain, R-40492 = LMG 25438 = CAIM 1454) and sp. nov. (type strain, R-40493 = LMG 25439 = CAIM 1455).

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2011-12-01
2019-12-05
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Supplements

vol. , part 12, pp. 3009 - 3015

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (525 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (743 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (556 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (790 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (553 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (531 bp).

Neighbour-joining phylogenetic tree showing the position of sp. nov. and sp. nov. based on gene sequences (830 bp).

Maximum-parsimony phylogenetic tree showing the position sp. nov. and sp. nov. based on 16S rRNA gene sequences.

GenBank accession numbers of the housekeeping gene sequences used in this study.

Sequence similarity values (%) between the , , , , , and gene sequences of sp. nov. R-40492 , V. maritimus sp. nov. R-40493 and the type strains of phylogenetically related species.

Cellular fatty acid contents of sp. nov., sp. nov. and phylogenetically related species.

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