1887

Abstract

Three novel aerobic, elliptic bacteria, designated DQHS4, DQHS8 and DQHS15, were isolated from sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. Cells were Gram-, oxidase- and catalase-negative. All three strains grew at 15–42 °C, pH 5–9 and NaCl concentrations between 0.5 and 10 %. DNA–DNA hybridization experiments revealed high (>85 %) relatedness among the three novel isolates and suggested that the strains constitute a single species. Comparative 16S rRNA gene sequence analysis indicated that these bacteria had less than 90 % similarity to all described species of the phylum ; the closest relative of the three isolates was F2, sharing only 89.6 % sequence similarity. The major cellular fatty acids were iso-C 3-OH (19.8–20.0 %), iso-C (16.9–17.3 %), anteiso-C B and/or iso-C I (7.4–8.7 %), C 2-OH (8.4 %), anteiso-C (8.2–8.6 %) and Cω6 (5.6–6.0 %). The major respiratory quinone was menaquinone-7 (MK-7) and the DNA G+C content was 41.8–43.5 mol%. Based on the distinct phylogenetic position and the combination of genotypic, phenotypic and chemotaxonomic characteristics, these three strains were considered to represent a novel species of a new genus in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is DQHS4 ( = CGMCC 1.9156  = NCCB 100301  = LMG 25367).

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2011-12-01
2019-10-23
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Supplements

vol. , part 12, pp. 2885 - 2889

Phylogenetic tree based on 16S rRNA gene sequences using the minimum-evolution method, showing the positions of strains DQHS4 , DQHS8 and DQHS15 and type strains of related taxa within the phylum .

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences using the heuristic search option, showing the positions of strains DQHS4 , DQHS8 and DQHS15 and type strains of related taxa within the phylum .

Dendrogram based on UPGMA cluster analysis of BOX-PCR genomic DNA fingerprints of strains DQHS4 , DQHS8 and DQHS15.

Transmission electron micrograph of a negatively stained cell of strain DQHS4 .

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