1887

Abstract

A taxonomic study was carried out on two bacterial strains, PCP11 and PCP104, isolated from a tidal flat of the Yellow Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these strains belonged to the family , phylum . Strains PCP11 and PCP104 shared 99.4 % sequence similarity and were related most closely to KMM 3525 (95.8 and 96.0 % sequence similarity, respectively). Members of the genera , , and were the next closest relatives of the new isolates, with sequence similarities ≤91 %. The two isolates were Gram-staining-negative, strictly aerobic, gliding bacteria. They grew in the presence of 1–5 % NaCl, at pH 5.5–8.5 and at 4–35 °C. Strains PCP11 and PCP104 shared a number of physiological and biochemical properties with KMM 3525, but they differed from this strain in the hydrolysis of biopolymers and in the production of carotenoid and flexirubin-type pigments. Both strains possessed iso-C, summed feature 4 (Cω7 and/or iso-C 2-OH) and C as major cellular fatty acids. The major respiratory quinone was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains PCP11 and PCP104 were 39.6 and 41.9 mol%, respectively. On the basis of phenotypic data and phylogenetic inference, it is proposed that the two isolates represent a novel species, sp. nov., with strain PCP11 ( = KACC 14523  = JCM 16588) as the type strain. Emended descriptions of the genus and are also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.026849-0
2011-08-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1994.html?itemId=/content/journal/ijsem/10.1099/ijs.0.026849-0&mimeType=html&fmt=ahah

References

  1. Agarwal S. , Hunnicutt D. W. , McBride M. J. . ( 1997; ). Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA . . Proc Natl Acad Sci U S A 94:, 12139–12144. [CrossRef].[PubMed].
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef].[PubMed].
    [Google Scholar]
  3. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef].[PubMed].
    [Google Scholar]
  4. Euzéby J. P. . ( 1997; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592. http://www.bacterio.cict.fr [CrossRef].[PubMed].
    [Google Scholar]
  5. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  6. González J. M. , Mayer F. , Moran M. A. , Hodson R. E. , Whitman W. B. . ( 1997; ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. . Int J Syst Bacteriol 47:, 369–376. [CrossRef].[PubMed].
    [Google Scholar]
  7. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  8. Hiraishi A. , Ueda Y. , Ishihara J. , Mori T. . ( 1996; ). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42:, 457–469. [CrossRef].
    [Google Scholar]
  9. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University;.[CrossRef]
    [Google Scholar]
  10. Ludwig W. , Euzéby J. , Whitman W. B. . ( 2008; ). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes . . http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
  11. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef].
    [Google Scholar]
  12. Nedashkovskaya O. I. , Suzuki M. , Vysotskii M. V. , Mikhailov V. V. . ( 2003; ). Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . . Int J Syst Evol Microbiol 53:, 81–85. [CrossRef].[PubMed].
    [Google Scholar]
  13. Nedashkovskaya O. I. , Kim S. B. , Suzuki M. , Shevchenko L. S. , Lee M. S. , Lee K. H. , Park M. S. , Frolova G. M. , Oh H. W. et al. ( 2005; ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55:, 2583–2588. [CrossRef].[PubMed].
    [Google Scholar]
  14. Park S. J. , Kang C. H. , Rhee S. K. . ( 2006; ). Characterization of the microbial diversity in a Korean solar saltern by 16S rRNA gene analysis. . J Microbiol Biotechnol 16:, 1640–1645.
    [Google Scholar]
  15. Reichenbach H. . ( 1989; ). The order Cytophagales Leadbetter 1974, 99AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 2011–2073. Edited by Staley J. T. , Bryant M. P. , Pfennig N. , Holt J. C. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  16. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed].
    [Google Scholar]
  17. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed].
    [Google Scholar]
  20. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed].
    [Google Scholar]
  21. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.026849-0
Loading
/content/journal/ijsem/10.1099/ijs.0.026849-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error