1887

Abstract

A novel Gram-staining-positive, moderately halophilic, facultatively alkaliphilic, non-motile, catalase-positive, oxidase-negative, endospore-forming, facultatively anaerobic rod, designated JSM 076093, was isolated from a sea urchin () collected from Naozhou Island in the South China Sea. Growth occurred with 0.5–25 % (w/v) NaCl (optimum 5–8 %) and at pH 6.0–10.5 (optimum pH 8.0) and 5–40 °C (optimum 30–35 °C). -Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The major cellular fatty acids (>10 % of the total) were anteiso-C, anteiso-C, iso-C and iso-C. The genomic DNA G+C content was 38.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076093 belonged to the genus and was related most closely to SW-72 (99.1 % 16S rRNA gene sequence similarity) and KMM 3737 (97.3 %). The combination of results from the phylogenetic analysis, DNA–DNA hybridization and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 076093 represents a novel species of the genus , for which the name sp. nov. is proposed, with JSM 076093 ( = DSM 23007 = KCTC 13710) as the type strain.

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2011-12-01
2019-12-08
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vol. , part 12, pp. 2950 - 2955

Phylogenetic trees showing the positions of sp. nov. JSM 076093 and closely related members of the genus , inferred from 16S rRNA gene sequences and constructed using maximum-likelihood and maximum-parsimony methods. Polar lipid profile of sp. nov. JSM 076093 analysed using two-dimensional TLC.

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