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Abstract

A bacterial strain designated KTW-12 was isolated from a reef-building coral in Kenting, Taiwan, and was characterized using a polyphasic taxonomic approach. Strain KTW-12 was Gram-negative, semi-transparent, slightly curved rod-shaped, and non-motile. Growth occurred at 15–35 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0), and with 0.5–6.0 % NaCl (optimum, 2 %). The major cellular fatty acids were summed feature 3 (Cω7 and/or Cω6), C and C. The DNA G+C content was 47.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KTW-12 was most closely related to MSSRF30, with 94.8 % gene sequence similarity. Further multilocus sequence analysis using , and genes also revealed low levels of sequence similarity (74.6–85.0 %) with all species of the genus with validly published names. A multigene phylogenetic tree using concatenated sequences of the four genes (16S rRNA, , and ) elucidated that strain KTW-12 occupied a distinct phylogenetic position, forming a long branch that was not clustered with any other known species of the genus . Strain KTW-12 differed from MSSRF30 in the ability to reduce nitrate to nitrite, hydrolysis of chitin, fermentation of sorbitol and production of arginine dihydrolase, valine arylamidase, cystine arylamidase and -acetyl-β-glucosaminidase. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain KTW-12 should be classified as representing a novel species, for which the name sp. nov. is proposed. The type strain is KTW-12 ( = BCRC 80105  = LMG 25357).

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2011-09-01
2019-10-20
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Supplements

vol. , part 9, pp. 2180 - 2185

[ PDF] (125 KB) containing:

Amplification and sequencing primers for , and genes

GN2 microplate oxidation data that can be used to distinguish strain KTW-12 from

Characteristics useful for differentiating strain KTW-12 from closely related species of the genus

Expanded phylogenetic tree based on 16S rRNA gene sequences of strain KTW-12 and related taxa

Neighbour-joining tree based on partial gene sequences of strain KTW-12 and other related organisms of the family

Neighbour-joining tree based on partial gene sequences of strain KTW-12 and other related organisms of the family

Neighbour-joining tree based on partial gene sequences of strain KTW-12 and other related organisms of the family



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