1887

Abstract

A Gram-negative, non-spore-forming, ovoid to rod-shaped aerobic or microaerobic bacterium, strain 262-8, was isolated from a cavity within white rock collected in Antarctica. Strain 262-8 grew at 5–30 °C (optimum 25 °C), at pH 6–8 (optimum approximately pH 7) and with 0.1–2.0 % (w/v) NaCl (optimum 0.5 % NaCl). The addition of tryptone or yeast extract was essential for growth. Strain 262-8 was able to utilize organic compounds such as ribose, pyruvate and succinate in the presence of a low concentration of tryptone. Ubiquinone 10 was the major respiratory quinone. The major fatty acids were C, C and C. The G+C content of the genomic DNA was 69.8 mol%. Comparative analyses of 16S rRNA gene sequences and physiological characteristics indicated that strain 262-8 was a phylogenetically novel bacterium that should be classified in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is 262-8 ( = JCM 16422 = ATCC BAA-1906).

Funding
This study was supported by the:
  • , JSPS KAKENHI , (Award 19205022)
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2011-08-01
2020-10-01
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References

  1. Alarico S., Rainey F. A., Empadinhas N., Schumann P., Nobre M. F., da Costa M. S. 2002; Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the alpha-1 subclass of the Proteobacteria . Syst Appl Microbiol 25:198–206 [CrossRef][PubMed]
    [Google Scholar]
  2. Bargagli R. 2005; Antarctica: geomorphology and climate trends. In Antarctic Ecosystems: Environmental Contamination, Climate Change and Human Impact pp. 1–42 Berlin: Springer-Verlag;
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef][PubMed]
    [Google Scholar]
  4. de la Torre J. R., Goebel B. M., Friedmann E. I., Pace N. R. 2003; Microbial diversity of cryptoendolithic communities from the McMurdo Dry Valleys, Antarctica. Appl Environ Microbiol 69:3858–3867 [CrossRef][PubMed]
    [Google Scholar]
  5. Fritz I., Strömpl C., Abraham W. R. 2004; Phylogenetic relationships of the genera Stella, Labrys and Angulomicrobium within the ‘Alphaproteobacteria’ and description of Angulomicrobium amanitiforme sp. nov.. Int J Syst Evol Microbiol 54:651–657 [CrossRef][PubMed]
    [Google Scholar]
  6. Gallego V., Sánchez-Porro C., García M. T., Ventosa A. 2006; Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56:2291–2295 [CrossRef][PubMed]
    [Google Scholar]
  7. Garrity M. G., Bell J. A., Lilburn T. 2005; Family I, Rhodospirillaceae Pfenning and Trüper 1971, 17AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 1–40 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  8. Guyoneaud R., Mouné S., Eatock C., Bothorel V., Hirschler-Réa A., Willison J., Duran R., Liesack W., Herbert R. et al. 2002; Characterization of three spiral-shaped purple nonsulfur bacteria isolated from coastal lagoon sediments, saline sulfur springs, and microbial mats: emended description of the genus Roseospira and description of Roseospira marina sp. nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov.. Arch Microbiol 178:315–324 [CrossRef][PubMed]
    [Google Scholar]
  9. Hiraishi A., Matsuzawa Y., Kanbe T., Wakao N. 2000; Acidisphaera rubrifaciens gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium isolated from acidic environments. Int J Syst Evol Microbiol 50:1539–1546 [CrossRef][PubMed]
    [Google Scholar]
  10. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic. Institute and State University Anaerobe Laboratory;
    [Google Scholar]
  11. Hughes K. A., Lawley B. 2003; A novel Antarctic microbial endolithic community within gypsum crusts. Environ Microbiol 5:555–565 [CrossRef][PubMed]
    [Google Scholar]
  12. Imhoff J. F. 2005a; Genus I, Rhodospirillum Molisch 1907, 24AL emend. Imhoff, Petri, and Süling 1998, 796VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 1–6 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  13. Imhoff J. F. 2005b; Genus IX, Roseospira Imhoff, Petri, and Süling 1998, 797VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 37–38 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  14. Imhoff J. F. 2005c; Genus V, Phaeospirillum Imhoff, Petri, and Süling 1998, 796VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 32–33 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  15. Imhoff J. F. 2005d; Genus VI, Rhodocista Kawasaki, Hoshino, Kuraishi, and Yamasato 1994b, 182VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 33–35 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  16. Imhoff J. F. 2005e; Genus VIII, Rhodovibrio Imhoff, Petri, and Süling 1998, 797VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 36–37 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  17. Karr E. A., Ng J. M., Belchik S. M., Sattley W. M., Madigan M. T., Achenbach L. A. 2006; Biodiversity of methanogenic and other archaea in the permanently frozen Lake Fryxell, Antarctica. Appl Environ Microbiol 72:1663–1666 [CrossRef][PubMed]
    [Google Scholar]
  18. Kishimoto N., Kosako Y., Wakao N., Tano T., Hiraishi A. 1995; Transfer of Acidiphilium facilis and Acidiphilium aminolytica to the genus Acidocella gen. nov., and emendation of the genus Acidiphilium . Syst Appl Microbiol 18:85–91 [CrossRef]
    [Google Scholar]
  19. Loganathan P., Nair S. 2004; Swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 54:1185–1190 [CrossRef][PubMed]
    [Google Scholar]
  20. Madigan M. T., Imhoff J. F. 2005; Genus XI, Rhodopila Imhoff, Trüper, and Pfennig 1984, 341VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 83–85 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  21. Pfennig N., Lünsdorf H., Süling J., Imhoff J. F. 1997; Rhodospira trueperi gen. nov., spec. nov., a new phototrophic proteobacterium of the alpha group. Arch Microbiol 168:39–45 [CrossRef][PubMed]
    [Google Scholar]
  22. Prisco G. D. 2007; Lake Vostok and subglacial lakes of Antarctica: do they host life?. In Physiology and Biochemistry of Extremophiles pp. 145–154 Edited by Gerday C., Glansdorff N. New York: American Society for Microbiology;
    [Google Scholar]
  23. Priscu J. C., Fritsen C. H., Adams E. E., Giovannoni S. J., Paerl H. W., McKay C. P., Doran P. T., Gordon D. A., Lanoil B. D., Pinckney J. L. 1998; Perennial Antarctic lake ice: an oasis for life in a polar desert. Science 280:2095–2098 [CrossRef][PubMed]
    [Google Scholar]
  24. Saitoh S., Suzuki T., Nishimura Y. 1998; Proposal of Craurococcus roseus gen. nov., sp. nov. and Paracraurococcus ruber gen. nov., sp. nov., novel aerobic bacteriochlorophyll a-containing bacteria from soil. Int J Syst Bacteriol 48:1043–1047 [CrossRef][PubMed]
    [Google Scholar]
  25. Schüler D., Schleifer K. H. 2005; Genus IV, Magnetospirillum Schleifer, Schüler, and Ludwig 1992, 291VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 28–31 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  26. Shi B. H., Arunpairojana V., Palakawong S., Yokota A. 2002; Tistrella mobilis gen nov, sp nov, a novel polyhydroxyalkanoate-producing bacterium belonging to α-Proteobacteria . J Gen Appl Microbiol 48:335–343 [CrossRef][PubMed]
    [Google Scholar]
  27. Sievers M., Swings J. 2005a; Family II, Acetobacteraceae Gillis and De Ley 1980, 23VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 41–96 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  28. Sievers M., Swings J. 2005b; Genus I, Acetobacter Beijerinck 1898, 215AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 51–54 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  29. Sievers M., Swings J. 2005c; Genus IX, Gluconobacter Asai 1935, 689AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 77–81 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  30. Sievers M., Swings J. 2005d; Genus VIII, Gluconacetobacter Yamada, Hoshino, and Ishikawa 1998b, 32VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 72–73 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  31. Sizova M. V., Panikov N. S., Spiridonova E. M., Slobodova N. V., Tourova T. P. 2007; Novel facultative anaerobic acidotolerant Telmatospirillum siberiense gen. nov. sp. nov. isolated from mesotrophic fen. Syst Appl Microbiol 30:213–220 [CrossRef][PubMed]
    [Google Scholar]
  32. Sly L. I., Stackebrandt E. 1999; Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum . Int J Syst Bacteriol 49:541–544 [CrossRef]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  35. Tindall B. J. 2004; Prokaryotic diversity in the Antarctic: the tip of the iceberg. Microb Ecol 47:271–283 [CrossRef][PubMed]
    [Google Scholar]
  36. Urakami T., Tamaoka J., Suzuki K., Komagata K. 1989; Acidomonas gen. nov., incorporating Acetobacter methanolicus as Acidomonas methanolica comb. nov.. Int J Syst Bacteriol 39:50–55 [CrossRef]
    [Google Scholar]
  37. Vasilyeva L. V. 2005; Genus XIV, Stella Vasilyeva 1985, 520VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 93–95 Edited by Brenner D. J., Kreig N. R. Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  38. Weon H.-Y., Kim B.-Y., Hong S.-B., Joa J.-H., Nam S.-S., Lee K. H., Kwon S.-W. 2007; Skermanella aerolata sp. nov., isolated from air, and emended description of the genus Skermanella . Int J Syst Evol Microbiol 57:1539–1542 [CrossRef][PubMed]
    [Google Scholar]
  39. Weyant R. S., Whitney A. M. 2005; Genus XIII, Roseomonas Rhis, Brenner, Weaver, Steigerwalt, Hollis, and Yu 1998, 627VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2C pp. 88–92 Edited by Brenner D. J., Kreig N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  40. Yamada Y., Katsura K., Kawasaki H., Widyastuti Y., Saono S., Seki T., Uchimura T., Komagata K. 2000; Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the α-Proteobacteria . Int J Syst Evol Microbiol 50:823–829 [CrossRef][PubMed]
    [Google Scholar]
  41. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad’on N., Gorlenko V. M., Kompantseva E. I., Drews G. 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:427–434 [CrossRef][PubMed]
    [Google Scholar]
  42. Zhang G. I., Hwang C. Y., Cho B. C. 2008; Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58:479–485 [CrossRef][PubMed]
    [Google Scholar]
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