1887

Abstract

Four bacterial strains isolated from root nodules of , and plants grown in the Yunnan province of China were identified as a lineage within the genus according to the analysis of 16S rRNA gene sequences, sharing most similarity with P1-7 (99.1 % sequence similarity) and IAM 13570 (99.0 %). These strains also formed a distinctive group from the reference strains for defined species of the genus in a polyphasic approach, including the phylogenetic analyses of the 16S rRNA gene and housekeeping genes (), DNA–DNA hybridization, BOX-PCR fingerprinting, phenotypic characterization, SDS-PAGE of whole-cell proteins, and cellular fatty acid profiles. All the data obtained in this study suggested that these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The DNA G+C content (mol%) of this species varied between 60.9 and 61.2 ( ). The type strain of sp. nov. is CCBAU 65647 ( = LMG 25295  = HAMBI 3073), which has a DNA G+C content of 60.9 mol% and forms effective nodules on .

Funding
This study was supported by the:
  • , State Key Basic Research and Development Plan of China , (Award 2006CB100206)
  • , National Program for Basic S & T Platform Construction , (Award 2005DKA21201-10)
  • , National Natural Science Foundation of China , (Award 30400001 and 30670001)
  • , IPN, Mexico , (Award SIP20100067) and SIP20090179)
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2011-11-01
2020-10-28
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