1887

Abstract

The sheathed filamentous bacterium known as strain CT3, isolated by micromanipulation from an activated sludge treatment plant in Italy, is a member of the genus in the - according to 16S rDNA sequence analysis. The closest phylogenetic neighbours of strain CT3 are strains I and Q, which were also isolated from activated sludge and belong to the species . These strains have respectively 99·2 and 99·4 % similarity to CT3 by 16S rDNA sequence comparison. CT3 shows 63–67 % DNA–DNA hybridization with strain I, which is the only currently viable strain of . CT3 is the second strain in the genus that has been shown to be capable of growing autotrophically with reduced sulfur compounds as the sole energy source; autotrophy was also confirmed in strain I. The first reported chemolithoautotrophic isolate of this genus was a strain of ‘’ that was isolated from a hydrogen sulfide spring and is morphologically distinguishable from all other described strains of , including CT3. CT3 is an aerobic organism that is non-fermentative, not capable of denitrification and able to grow heterotrophically. Autotrophy in the genus should be investigated more fully to better define the taxonomy of this genus.

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2003-09-01
2019-10-23
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vol. , part 5, pp. 1271 - 1276

Micrographs of strain CT3, showing (a) presence of sheath (arrowed); (b) appearance of filaments that contain sulfur granules; (c) production of gonidia from filament apex (arrowed); (d) typical organization in rosette-like array; (e) bright fluorescent lipophilic granules following Nile blue staining. (f) Appearance of macroscopic growth of CT3 (left) and strain I (right) in cultivation medium at 20 ºC. Bars, 5 µm. [PDF](667 KB)



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