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Four coagulase-negative, novobiocin-resistant cocci, designated CW1T, PM34, MM3 and RW78, were isolated from the respiratory tract of goats kept in the Himalayan region. The four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after SDS-PAGE and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (ERIC)-PCR. Strain CW1T showed highest 16S rDNA sequence similarities to Staphylococcus cohnii subsp. urealyticus ATCC 49330T, Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305T, S. cohnii subsp. cohnii ATCC 29974T, Staphylococcus arlettae ATCC 43957T, Staphylococcus gallinarum ATCC 35539T, Staphylococcus succinus ATCC 700337T and Staphylococcus xylosus ATCC 29971T (99·0, 98·8, 98·8, 98·4, 98·2, 98·1 and 98·1 %, respectively), indicating its classification within the genus Staphylococcus. The polar lipid composition, fatty acid profiles, quinone systems and diagnostic cell-wall diamino acid were in agreement with the characteristics of the genus Staphylococcus. DNA–DNA hybridization with closely related Staphylococcus species suggested that strain CW1T represents an as-yet unrecognized species. Based on these results, a novel species of the genus Staphylococcus is described, Staphylococcus nepalensis sp. nov. The type strain is CW1T (=DSM 15150T=CCM 7045T) and the most dissimilar strain is PM34 (=DSM 15151=CCM 7046).
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International Journal of Systematic and Evolutionary Microbiology vol. 53 , part 6, pp. 2007 - 2011
Supplementary Figure. SDS-PAGE of whole-cell lysates of Staphylococcus species. Lanes: 1 and 14, molecular mass standard; 2, S. succinus subsp. succinus ATCC 700337 T; 3, S. kloosii CCM 3834 T; 4, S. lugdunensis CCM 4064 T; 5, S. arlettae CCM 3830 T; 6, S. cohnii subsp. cohnii CCM 2736 T; 7, S. cohnii subsp. urealyticum CCM 4294 T; 8 and 15, S. nepalensis CW1 T; 9, S. saprophyticus subsp. saprophyticus CCM 883 T; 10, S. xylosus DSM 20231 T; 11, S. equorum CCM 3832 T; 12, S. haemolyticus DSM 20263 T; 13, S. gallinarum DSM 20610 T; 1618, S. nepalensis strains PM34, MM3 and RW78.