1887

Abstract

Four coagulase-negative, novobiocin-resistant cocci, designated CW1, PM34, MM3 and RW78, were isolated from the respiratory tract of goats kept in the Himalayan region. The four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after SDS-PAGE and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (ERIC)-PCR. Strain CW1 showed highest 16S rDNA sequence similarities to subsp. ATCC 49330, subsp. ATCC 15305, subsp. ATCC 29974, ATCC 43957, ATCC 35539, ATCC 700337 and ATCC 29971 (99·0, 98·8, 98·8, 98·4, 98·2, 98·1 and 98·1 %, respectively), indicating its classification within the genus . The polar lipid composition, fatty acid profiles, quinone systems and diagnostic cell-wall diamino acid were in agreement with the characteristics of the genus . DNA–DNA hybridization with closely related species suggested that strain CW1 represents an as-yet unrecognized species. Based on these results, a novel species of the genus is described, sp. nov. The type strain is CW1 (=DSM 15150=CCM 7045) and the most dissimilar strain is PM34 (=DSM 15151=CCM 7046).

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2003-11-01
2019-10-14
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vol. , part 6, pp. 2007 - 2011

SDS-PAGE of whole-cell lysates of species. Lanes: 1 and 14, molecular mass standard; 2, subsp. ATCC 700337 ; 3, CCM 3834 ; 4, CCM 4064 ; 5, CCM 3830 ; 6, subsp. CCM 2736 ; 7, subsp. CCM 4294 ; 8 and 15, CW1 ; 9, subsp. CCM 883 ; 10, DSM 20231 ; 11, CCM 3832 ; 12, DSM 20263 ; 13, DSM 20610 ; 16–18, strains PM34, MM3 and RW78.



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