1887

Abstract

A novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2, was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the ‘Sarah Spring’ area (7° 25′ 24″ S 107° 47′ 66″ W). Cells were irregular, motile cocci (0.8–1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2 grew at salt concentrations of 1–5 % (w/v) NaCl with an optimum at 2 %. The novel isolate grew by fermentation or sulphur respiration on a variety of organic compounds. It was a chemoorganoheterotrophic archaeon growing preferentially with yeast extract, peptone and tryptone as carbon and energy sources and sulphur and organic compounds as electron acceptors; it also grew on maltose and starch. Sulphur or -cystine were required for growth and were reduced to hydrogen sulfide. The strain was resistant to rifampicin, chloramphenicol, vancomycin and kanamycin (all at 100 µg ml) but was sensitive to tetracycline. The G+C content of its genomic DNA was 53.6 mol%. Phylogenetic analysis of the almost complete 16S rRNA gene sequence (1450 bp) of strain Bio-pl-0405IT2 showed that the novel isolate belonged to the genus DNA–DNA hybridization values with the two closest relatives AL662 and JCM 8558 were below the threshold value of 70 %. On the basis of the physiological and genotypic distinctness, we propose a novel species, sp. nov. The type strain is Bio-pl-0405IT2 ( = CSUR P577 = JCM 16307).

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2013-08-01
2019-09-22
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References

  1. Achenbach-Richter L. , Gupta R. , Zillig W. , Woese C. R. . ( 1988; ). Rooting the archaebacterial tree: the pivotal role of Thermococcus celer in archaebacterial evolution. . Syst Appl Microbiol 10:, 231–240. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  3. Balch W. E. , Wolfe R. S. . ( 1976; ). New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. . Appl Environ Microbiol 32:, 781–791.[PubMed]
    [Google Scholar]
  4. Benbouzid-Rollet N. , López-García P. , Watrin L. , Erauso G. , Prieur D. , Forterre P. . ( 1997; ). Isolation of new plasmids from hyperthermophilic Archaea of the order Thermococcales . . Res Microbiol 148:, 767–775. [CrossRef] [PubMed]
    [Google Scholar]
  5. Birnboim H. C. , Doly J. . ( 1979; ). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. . Nucleic Acids Res 7:, 1513–1523. [CrossRef] [PubMed]
    [Google Scholar]
  6. Canganella F. , Jones W. J. , Gambacorta A. , Antranikian G. . ( 1998; ). Thermococcus guaymasensis sp. nov. and Thermococcus aggregans sp. nov., two novel thermophilic archaea isolated from the Guaymas Basin hydrothermal vent site. . Int J Syst Bacteriol 48:, 1181–1185. [CrossRef] [PubMed]
    [Google Scholar]
  7. Cord-Ruwisch R. . ( 1985; ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. . J Microbiol Methods 4:, 33–36. [CrossRef]
    [Google Scholar]
  8. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  9. Erauso G. , Marsin S. , Benbouzid-Rollet N. , Baucher M.-F. , Barbeyron T. , Zivanovic Y. , Prieur D. , Forterre P. . ( 1996; ). Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: evidence for rolling-circle replication in a hyperthermophile. . J Bacteriol 178:, 3232–3237.[PubMed]
    [Google Scholar]
  10. Geslin C. , Le Romancer M. , Erauso G. , Gaillard M. , Perrot G. , Prieur D. . ( 2003; ). PAV1, the first virus-like particle isolated from a hyperthermophilic euryarchaeote, “Pyrococcus abyssi”. . J Bacteriol 185:, 3888–3894. [CrossRef] [PubMed]
    [Google Scholar]
  11. Geslin C. , Gaillard M. , Flament D. , Rouault K. , Le Romancer M. , Prieur D. , Erauso G. . ( 2007; ). Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi. . J Bacteriol 189:, 4510–4519. [CrossRef] [PubMed]
    [Google Scholar]
  12. Godfroy A. , Lesongeur F. , Raguénès G. , Quérellou J. , Antoine E. , Meunier J.-R. , Guezennec J. , Barbier G. . ( 1997; ). Thermococcus hydrothermalis sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. . Int J Syst Bacteriol 47:, 622–626. [CrossRef] [PubMed]
    [Google Scholar]
  13. Gonnet M. , Erauso G. , Prieur D. , Le Romancer M. . ( 2011; ). pAMT11, a novel plasmid isolated from a Thermococcus sp. strain closely related to the virus-like integrated element TKV1 of the Thermococcus kodakaraensis genome. . Res Microbiol 162:, 132–143. [CrossRef] [PubMed]
    [Google Scholar]
  14. Gorlas A. , Koonin E. V. , Bienvenu N. , Prieur D. , Geslin C. . ( 2012; ). TPV1, the first virus isolated from the hyperthermophilic genus Thermococcus . . Environ Microbiol 14:, 503–516. [CrossRef] [PubMed]
    [Google Scholar]
  15. Grote R. , Li L. , Tamaoka J. , Kato C. , Horikoshi K. , Antranikian G. . ( 1999; ). Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. . Extremophiles 3:, 55–62. [CrossRef] [PubMed]
    [Google Scholar]
  16. Honda D. , Inouye I. . ( 2002; ). Ultrastructure and taxonomy of a marine photosynthetic stramenopile Phaeomonas parva gen. et sp. nov. (Pinguiophyceae) with emphasis on the flagellar apparatus architecture. . Phycol Res 50:, 75–89. [CrossRef]
    [Google Scholar]
  17. Huss V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  18. Jolivet E. , L’Haridon S. , Corre E. , Forterre P. , Prieur D. . ( 2003; ). Thermococcus gammatolerans sp. nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation. . Int J Syst Evol Microbiol 53:, 847–851. [CrossRef] [PubMed]
    [Google Scholar]
  19. Marteinsson V.-T. , Birrien J.-L. , Reysenbach A.-L. , Vernet M. , Marie D. , Gambacorta A. , Messner P. , Sleytr U.-B. , Prieur D. . ( 1999; ). Thermococcus barophilus sp. nov., a new barophilic and hyperthermophilic archaeon isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. . Int J Syst Bacteriol 49:, 351–359. [CrossRef] [PubMed]
    [Google Scholar]
  20. Prieur D. . ( 2002; ). Hydrothermal vents: prokaryotes in deep sea hydrothermal vents. . In Encyclopedia of Environmental Microbiology, pp. 1617–1628. Edited by Bitton G. . . New York:: John Wiley & Sons;.
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Soler N. , Justome A. , Quevillon-Cheruel S. , Lorieux F. , Le Cam E. , Marguet E. , Forterre P. . ( 2007; ). The rolling-circle plasmid pTN1 from the hyperthermophilic archaeon Thermococcus nautilus . . Mol Microbiol 66:, 357–370. [CrossRef] [PubMed]
    [Google Scholar]
  23. Soler N. , Marguet E. , Cortez D. , Desnoues N. , Keller J. , van Tilbeurgh H. , Sezonov G. , Forterre P. . ( 2010; ). Two novel families of plasmids from hyperthermophilic archaea encoding new families of replication proteins. . Nucleic Acids Res 38:, 5088–5104. [CrossRef] [PubMed]
    [Google Scholar]
  24. Stetter K. O. . ( 1996; ). Hyperthermophilic prokaryotes. . FEMS Microbiol Rev 18:, 149–158. [CrossRef]
    [Google Scholar]
  25. Takai K. , Horikoshi K. . ( 2000; ). Thermosipho japonicus sp. nov., an extremely thermophilic bacterium isolated from a deep-sea hydrothermal vent in Japan. . Extremophiles 4:, 9–17.[PubMed] [CrossRef]
    [Google Scholar]
  26. Takai K. , Sako Y. . ( 1999; ). A molecular view of archaeal diversity in marine and terrestrial hot water environments. . FEMS Microbiol Lett 28:, 177–188. [CrossRef]
    [Google Scholar]
  27. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  28. Ward D. E. , Revet I. M. , Nandakumar R. , Tuttle J. H. , de Vos W. M. , van der Oost J. , DiRuggiero J. . ( 2002; ). Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. . J Bacteriol 184:, 2561–2566. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconstitution of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  30. Zillig W. . ( 1992; ). The order Thermococcales . . In The Prokaryotes, , 2nd edn., vol. I, pp. 702–706. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K.-H. . . New York:: Springer;.
    [Google Scholar]
  31. Zillig W. , Holz I. , Janekovic D. , Schäfer W. , Reiter W. D. . ( 1983; ). The archaebacterium Thermococcus celer represents a novel genus within the thermophilic branch of the archaebacteria. . Syst Appl Microbiol 4:, 88–94. [CrossRef] [PubMed]
    [Google Scholar]
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