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Abstract

A Gram-stain-positive, non-motile actinomycete, designated strain YIM 48875, was isolated from rhizosphere soil of and its taxonomic position was established by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 48875 belonged to the genus , supported by a bootstrap value of 100 %. Cells of strain YIM 48875 showed two kinds of sporangia, which also supported its classification in the genus. Strain YIM 48875 grew optimally at 28 °C, at pH 6.0–8.0 and in the presence of 2 % (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain YIM 48875 and YIM 46034 was 98.6 %. Strain YIM 48875 exhibited a quinone system with menaquinones MK-9(H), MK-9(H) and MK-9(H) as the predominant compounds, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol mannoside and the major fatty acids iso-C and iso-C; these data were markedly different from those for YIM 46034. The level of DNA–DNA relatedness between strain YIM 48875 and YIM 46034 was 45.5 %. It is apparent from the genotypic and phenotypic data that strain YIM 48875 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 48875 ( = CCTCC AA 209049  = KCTC 19779).

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2011-06-01
2019-10-20
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vol. , part 6, pp. 1330 - 1333

Two-dimensional thin-layer chromatogram showing the polar lipids of strain YIM 48875 . DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PIM, phosphatidylinositol mannoside.



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