1887

Abstract

Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01 and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced -lactic acid from glucose. They grew at pH 5.0–9.0, at 15–40 °C and in the presence of 10 % (w/v) NaCl. The dominant fatty acid was Cω9. The DNA G+C contents of strains C01 and C02 were 38.6 and 38.7 mol%, respectively. Strain C01 was related most closely to DSM 20581, with a 16S rRNA gene sequence similarity of 96.9 %. The strains could be distinguished clearly from DSM 20581 based on cell morphology, physiological and biochemical characteristics and low levels of DNA–DNA relatedness. On the basis of the data presented, strains C01 and C02 are considered to represent a novel species of a new genus in the cluster, for which the name gen. nov., sp. nov. is proposed. The type strain of is C01 ( = KCTC 13150  = TISTR 1958  = PCU 316).

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2011-07-01
2020-01-23
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vol. , part 7, pp. 1688 - 1692

Maximum-parsimony (Fig. S1) and minimum-evolution (Fig. S2) phylogenetic trees based on almost-complete 16S rRNA gene sequences showing the relationships between strain C01 and the type strains of related members of the family .

Comparison of 16S rRNA gene signature nucleotides of strain C01 and closely related genera.

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