1887

Abstract

A previously undescribed facultatively anaerobic, catalase-negative, -like bacterium was isolated from the nose of a human. On the basis of its cellular morphology and the results of biochemical testing, the micro-organism was tentatively identified as a member of the genus , but it did not correspond to any currently recognized species. Comparative 16S rRNA gene sequencing studies showed the bacterium to be a hitherto unknown subline within the genus , displaying sequence divergence values of more than 6 % with respect to recognized species of the genus. On the basis of biochemical, molecular chemical and molecular phylogenetic evidence, it is proposed that the unknown organism, strain R2014 (=CCUG 46092=CIP 107668), be classified as the type strain of a novel species, sp. nov.

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2003-09-01
2024-05-06
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References

  1. Buchanan A. M., Scott J. L., Gerencser M. A., Beaman B. L., Jang S., Biberstein E. L. 1984; Actinomyces hordeovulneris sp. nov., an agent of canine actinomycosis. Int J Syst Bacteriol 34:439–443 [CrossRef]
    [Google Scholar]
  2. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp 265–309Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  3. Collins M. D., Hoyles L., Kalfas S., Sundquist G., Monsen T., Nikolaitchouk N., Falsen E. 2000; Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov. J Clin Microbiol 38:3399–3403
    [Google Scholar]
  4. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2. Cladistics 5:164–166
    [Google Scholar]
  5. Funke G., Stubbs S., von Graevenitz A., Collins M. D. 1994; Assignment of human-derived CDC group 1 coryneform bacteria and CDC group 1-like coryneform bacteria to the genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov. and Actinomyces neuii subsp. anitratus subsp. nov. Int J Syst Bacteriol 44:167–171 [CrossRef]
    [Google Scholar]
  6. Funke G., Alvarez N., Pascual C., Falsen E., Akervall E., Sabbe L., Schouls L., Weiss N., Collins M. D. 1997; Actinomyces europaeus sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:687–692 [CrossRef]
    [Google Scholar]
  7. Hall V., O'Neill G. L., Magee J. T., Duerden B. I. 1999; Development of amplified 16S ribosomal DNA restriction analysis for identification of Actinomyces species and comparison with pyrolysis-mass spectrometry and conventional biochemical tests. J Clin Microbiol 37:2255–2261
    [Google Scholar]
  8. Hall V., Talbot P. R., Stubbs S. L., Duerden B. I. 2001; Identification of clinical isolates of Actinomyces species by amplified 16S ribosomal DNA restriction analysis. J Clin Microbiol 39:3555–3562 [CrossRef]
    [Google Scholar]
  9. Hall V., Collins M. D., Hutson R., Falsen E., Duerden B. I. 2002; Actinomyces cardiffensis sp. nov., from human clinical sources. J Clin Microbiol 40:3427–3431 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  11. Lawson P. A., Nikolaitchouk N., Falsen E., Westling K., Collins M. D. 2001; Actinomyces funkei sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51:853–855 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. 1997; GeneDoc: analysis and visualisation of genetic variation. EMBNEW News 4:14
    [Google Scholar]
  14. Nikolaitchouk N., Hoyles L., Falsen E., Grainger J. M., Collins M. D. 2000; Characterization of Actinomyces isolates from samples from the human urogenital tract: description of Actinomyces urogenitalis sp. nov. Int J Syst Evol Microbiol 50:1649–1654 [CrossRef]
    [Google Scholar]
  15. Pascual Ramos C., Falsen E., Alvarez N., Åkervall E., Sjödén B., Collins M. D. 1997; Actinomyces graevenitzii sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:885–888 [CrossRef]
    [Google Scholar]
  16. Phillips K. D. 1976; A simple and sensitive technique for determining the fermentation reactions of non-sporing anaerobes. J Appl Bacteriol 41:325–328 [CrossRef]
    [Google Scholar]
  17. Rasmussen S. W. 1995 DNATools, a Software Package for DNA Sequence Analysis Carlsberg Laboratory; Copenhagen, Denmark:
    [Google Scholar]
  18. Schaal K. P. 1992; The genera Actinomyces , Arcanobacterium , and Rothia . In The Prokaryotes , 2nd edn. vol 1 pp 850–905Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  20. Wüst J., Stubbs S., Weiss N., Funke G., Collins M. D. 1995; Assignment of Actinomyces pyogenes -like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turicensis sp. nov. Lett Appl Microbiol 2076–81 [CrossRef]
    [Google Scholar]
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