1887

Abstract

A study employing a polyphasic taxonomic approach was undertaken to clarify the position of 12 isolates recovered from sewage samples. These isolates were recognized as a potential novel species because a new and specific pattern was produced with the 16S rRNA-RFLP identification method. The sequences of the 16S rRNA gene not only supported the classification of these novel strains as members of the genus , but also showed that they formed a separate phylogenetic line. Strain SW28-11, chosen as the representative of these strains, showed 16S rRNA gene sequence similarity of 95.6 % with the closest related species . The phylogenetic position of the novel strains was further confirmed by analysis of the housekeeping genes , and, for the first time, . The latter proved to be an excellent additional gene for establishing the phylogeny of this genus. These data, together with phenotypic characterization, revealed that this group of isolates represent a novel species of the genus . The name sp. nov., is proposed, with the type strain SW28-11 ( = CECT 7697 = LMG 25694).

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2011-09-01
2019-09-15
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vol. , part 9, pp. 2155 - 2161

Features of all isolates recovered from wastewater treatment plant.

Agarose gel comparing the 16S rRNA-RFLP pattern of sp. nov. with those of represented by the two defined hybridization groups 1A and 1B and , which are the most similar (Figueras 2008).

Cluster analysis based on the patterns obtained by ERIC-PCR from the sp. nov. strains.

Neighbour-joining tree based on gene sequences showing the phylogenetic position of sp. nov. within the genus .

Neighbour-joining tree based on gene sequences showing the phylogenetic position of sp. nov. within the genus .

Neighbour-joining tree based on gene sequences showing the phylogenetic position of sp. nov. within the genus .

Images of cells of strain SW28-11 as observed with scanning electron microscopy and transmission electron microscopy with a negative staining.

Agarose gel showing the sizes of the m-PCR (Houf , 2000) products of sp. nov. and other species of the genus .

Agarose gel showing the amplicons obtained with the m-PCR (Douidah , 2010) for sp. nov. and other species of the genus .

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