1887

Abstract

A novel aerobic, chemoheterotrophic bacterium, strain YO-36, isolated from the rhizoplane of an aquatic plant (a reed, ) inhabiting a freshwater lake in Japan, was morphologically, physiologically and phylogenetically characterized. Strain YO-36 was Gram-negative and ovoid to rod-shaped, and formed pinkish hard colonies on agar plates. Strain YO-36 grew at 20–40 °C with optimum growth at 30–35 °C, whilst no growth was observed at 15 °C or 45 °C. The pH range for growth was 5.5–8.5 with an optimum at pH 6.5. Strain YO-36 utilized a limited range of substrates, such as sucrose, gentiobiose, pectin, gellan gum and xanthan gum. The strain contained C, C, C and C as the major cellular fatty acids and menaquinone-12 as the respiratory quinone. The G+C content of the genomic DNA was 62.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YO-36 belonged to the candidate phylum OP10 comprised solely of environmental 16S rRNA gene clone sequences except for two strains, P488 and T49 isolated from geothermal soil in New Zealand; strain YO-36 showed less than 80 % sequence similarity to strains P488 and T47. Based on the phylogetic and phenotypic findings, a new genus and species, gen. nov., sp. nov., is proposed for the isolate (type strain YO-36  = NBRC 105658  = DSM 23562). In addition, a new bacterial phylum named phyl. nov. is proposed for the candidate phylum OP10 represented by gen. nov., sp. nov. and fam. nov., ord. nov., and classis nov.

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2011-06-01
2019-12-11
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