1887

Abstract

Thirteen -like isolates from the environment (water, soil and activated sludge) and human clinical samples (including respiratory secretions of cystic fibrosis patients) were investigated in a polyphasic taxonomic study that employed 16S rDNA sequence analysis, DNA–DNA hybridization, determination of DNA base composition, whole-cell protein analysis, biochemical characterization and PCR-based assays. All isolates were classified as a novel species, for which the name sp. nov. is proposed. The type strain, LMG 21421 (=CCUG 46789), was isolated from the sputum of a patient with acute lymphoblastic leukaemia. can be differentiated from other species of the genus and phenotypically similar species (including the complex and ) by a variety of biochemical tests, whole-cell protein analysis and several PCR-based assays. Some outstanding issues in the taxonomy of the genus are also discussed.

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2003-07-01
2019-11-21
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vol. , part 4, pp. 1075 - 1080

Normalized whole-cell protein profiles and dendrogram

PCR analysis of strains with primer pair Rp-F1/R38R1

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