1887

Abstract

A Gram-stain-positive, rod-shaped, non-sporulating, motile and moderately halophilic bacterium, designated strain H96B60, was isolated from a saline soil sample of the Qaidam basin, China. The strain was facultatively anaerobic. Major end products formed from glucose fermentation were acetate, ethanol and lactic acid. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone component was menaquinone-6 (MK-6). The predominant cellular fatty acids were C, anteiso-C and anteiso-C. The genomic DNA G+C content of strain H96B60 was 36.2 mol%. Phylogenetic analysis based on comparative 16S rRNA gene sequences indicated that strain H96B60 represented a novel phyletic lineage within the family and was related most closely to species (96.1–96.4 % similarity). Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain H96B60 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is H96B60 ( = DSM 22440  = CGMCC 1.7733).

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2011-05-01
2019-10-21
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References

  1. Ahmed I. , Yokota A. , Fujiwara T. . ( 2007; ). Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 57:, 796–802. [CrossRef] [PubMed]
    [Google Scholar]
  2. An S. Y. , Ishikawa S. , Kasai H. , Goto K. , Yokota A. . ( 2007; ). Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan. . Int J Syst Evol Microbiol 57:, 2489–2492. [CrossRef] [PubMed]
    [Google Scholar]
  3. Ash C. , Farrow J. A. E. , Wallbanks S. , Collins M. D. . ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  4. Cao S. J. , Qu J. H. , Yang J. S. , Sun Q. , Yuan H. L. . ( 2008; ). Halolactibacillus alkaliphilus sp. nov., a moderately alkaliphilic and halophilic bacterium isolated from a soda lake in Inner Mongolia, China, and emended description of the genus Halolactibacillus . . Int J Syst Evol Microbiol 58:, 2169–2173. [CrossRef] [PubMed]
    [Google Scholar]
  5. Carrasco I. J. , Márquez M. C. , Yanfen X. , Ma Y. , Cowan D. A. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2006; ). Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 56:, 599–604. [CrossRef] [PubMed]
    [Google Scholar]
  6. Carrasco I. J. , Márquez M. C. , Xue Y. , Ma Y. , Cowan D. A. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2008; ). Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic, Gram-positive bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 58:, 1961–1967. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chen Y. G. , Cui X. L. , Zhang Y. Q. , Li W. J. , Wang Y. X. , Xu L. H. , Peng Q. , Wen M. L. , Jiang C. L. . ( 2008; ). Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil. . Int J Syst Evol Microbiol 58:, 2403–2408. [CrossRef] [PubMed]
    [Google Scholar]
  8. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  9. Duckworth A. W. , Grant W. D. , Jones B. E. , Steenbergen R. V. . ( 1996; ). Phylogenetic diversity of soda lake alkaliphiles. . FEMS Microbiol Ecol 19:, 181–191. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. García M. T. , Gallego V. , Ventosa A. , Mellado E. . ( 2005; ). Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium. . Int J Syst Evol Microbiol 55:, 1789–1795. [CrossRef] [PubMed]
    [Google Scholar]
  12. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef] [PubMed]
    [Google Scholar]
  13. Heyndrickx M. , Lebbe L. , Kersters K. , De Vos P. , Forsyth G. , Logan N. A. . ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . . Int J Syst Bacteriol 48:, 99–106. [CrossRef]
    [Google Scholar]
  14. Ishikawa M. , Ishizaki S. , Yamamoto Y. , Yamasato K. . ( 2002; ). Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. . J Gen Appl Microbiol 48:, 269–279. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ishikawa M. , Nakajima K. , Yanagi M. , Yamamoto Y. , Yamasato K. . ( 2003; ). Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan. . Int J Syst Evol Microbiol 53:, 711–720. [CrossRef] [PubMed]
    [Google Scholar]
  16. Ishikawa M. , Nakajima K. , Itamiya Y. , Furukawa S. , Yamamoto Y. , Yamasato K. . ( 2005; ). Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. . Int J Syst Evol Microbiol 55:, 2427–2439. [CrossRef] [PubMed]
    [Google Scholar]
  17. Jeffries C. D. , Holtman D. F. , Guse D. G. . ( 1957; ). Rapid method for determining the activity of microorganisms on nucleic acids. . J Bacteriol 73:, 590–591.[PubMed]
    [Google Scholar]
  18. Jeon C. O. , Lim J. M. , Jang H. H. , Park D. J. , Xu L. H. , Jiang C. L. , Kim C. J. . ( 2008; ). Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China. . Int J Syst Evol Microbiol 58:, 2282–2286. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  20. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kushner D. J. . ( 1985; ). The Halobacteriaceae . . In The Bacteria, a Treatise on Structure and Function, vol. VIII, pp. 171–206. Edited by Gunsalus I. C. , Woese C. R. , Wolfe R. S. . . San Diego:: Academic Press;.
    [Google Scholar]
  22. Kwon K. S. , Lee J. , Kang H. G. , Hah Y. C. . ( 1994; ). Detection of β-glucosidase activity in polyacrylamide gels with esculin as substrate. . Appl Environ Microbiol 60:, 4584–4586.[PubMed]
    [Google Scholar]
  23. Lu J. , Nogi Y. , Takami H. . ( 2001; ). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. . FEMS Microbiol Lett 205:, 291–297. [CrossRef] [PubMed]
    [Google Scholar]
  24. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  25. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  26. Nielsen P. , Rainey F. A. , Outtrup H. , Priest F. G. , Fritze D. . ( 1994; ). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus . . FEMS Microbiol Lett 117:, 61–65. [CrossRef]
    [Google Scholar]
  27. Niimura Y. , Koh E. , Yanagida F. , Suzuki K. , Komagata K. , Kozaki M. . ( 1990; ). Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. . Int J Syst Bacteriol 40:, 297–301. [CrossRef]
    [Google Scholar]
  28. Priest F. G. . ( 1981; ). DNA homology in the genus Bacillus . . In The Aerobic Endospore-forming Bacteria, pp. 33–57. Edited by Berkeley R. C. , Goodfellow M. . . London:: Academic Press;.
    [Google Scholar]
  29. Quesada E. , Ventosa A. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1984; ). Deleya halophila, a new species of moderately halophilic bacteria. . Int J Syst Bacteriol 34:, 287–292. [CrossRef]
    [Google Scholar]
  30. Ren P. G. , Zhou P. J. . ( 2005; ). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. . Int J Syst Evol Microbiol 55:, 95–99. [CrossRef] [PubMed]
    [Google Scholar]
  31. Ryan S. M. , Fitzgerald G. F. , van Sinderen D. . ( 2006; ). Screening for and identification of starch-, amylopectin-, and pullulan-degrading activities in bifidobacterial strains. . Appl Environ Microbiol 72:, 5289–5296. [CrossRef] [PubMed]
    [Google Scholar]
  32. Sánchez-Porro C. , Martín S. , Mellado E. , Ventosa A. . ( 2003; ). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. . J Appl Microbiol 94:, 295–300. [CrossRef] [PubMed]
    [Google Scholar]
  33. Saqib A. , Whitney P. J. . ( 2006; ). Esculin gel diffusion assay (EGDA): a simple and sensitive method for screening β-glucosidases. . Enzyme Microb Technol 39:, 182–184. [CrossRef]
    [Google Scholar]
  34. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI, Inc.
  35. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  36. Schlesner H. , Lawson P. A. , Collins M. D. , Weiss N. , Wehmeyer U. , Völker H. , Thomm M. . ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. . Int J Syst Evol Microbiol 51:, 425–431.[PubMed]
    [Google Scholar]
  37. Sehgal S. N. , Gibbons N. E. . ( 1960; ). Effect of some metal ions on the growth of Halobacterium cutirubrum . . Can J Microbiol 6:, 165–169. [CrossRef] [PubMed]
    [Google Scholar]
  38. Sorokin I. D. , Zadorina E. V. , Kravchenko I. K. , Boulygina E. S. , Tourova T. P. , Sorokin D. Y. . ( 2008; ). Natronobacillus azotifigens gen. nov., sp. nov., an anaerobic diazotrophic haloalkaliphile from soda-rich habitats. . Extremophiles 12:, 819–827. [CrossRef] [PubMed]
    [Google Scholar]
  39. Spring S. , Ludwig W. , Marquez M. C. , Ventosa A. , Schleifer K. H. . ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.. Int J Syst Bacteriol 46:, 492–496. [CrossRef]
    [Google Scholar]
  40. Stackebrandt E. , Liesack W. . ( 1993; ). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 152–189. Edited by Goodfellow M. , O’Donnell A. G. . . London:: Academic Press;.
    [Google Scholar]
  41. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef]
    [Google Scholar]
  42. Ventosa A. , Quesada E. , Rodriguez-Valera F. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  43. Ventosa A. , Nieto J. J. , Oren A. . ( 1998; ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  44. Wainø M. , Tindall B. J. , Schumann P. , Ingvorsen K. . ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:, 821–831. [CrossRef] [PubMed]
    [Google Scholar]
  45. Wang X. W. , Xue Y. F. , Ma Y. H. . ( 2009; ). Sediminibacillus albus sp. nov., a moderately halophilic, Gram-positive bacterium isolated from a hypersaline lake, and emended description of the genus Sediminibacillus Carrasco et al. 2008. . Int J Syst Evol Microbiol 59:, 1640–1644. [CrossRef] [PubMed]
    [Google Scholar]
  46. Yoon J. H. , Kang K. H. , Park Y. H. . ( 2002; ). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. . Int J Syst Evol Microbiol 52:, 2043–2048. [CrossRef] [PubMed]
    [Google Scholar]
  47. Zhilina T. N. , Garnova E. S. , Tourova T. P. , Kostrikina N. A. , Zavarzin G. A. . ( 2001; ). Amphibacillus fermentum sp. nov. and Amphibacillus tropicus sp. nov., new alkaliphilic, facultatively anaerobic, saccharolytic bacilli from Lake Magadi. . Microbiology (English translation of Mikrobiologiia) 70:, 711–722.
    [Google Scholar]
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vol. , part 5, pp. 1127 - 1132

Cellular fatty acid profiles of strain H96B60 and closely related members of the family .

Products from glucose under anaerobic fermentation conditions by strain H96B60 , M2-2 and M23-1 .

Phylogenetic trees based on the minimum-evolution and maximum-parsimony algorithms showing the relationships between strain H96B60 and some other related taxa based on 16S rRNA gene sequence analyses.

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