1887

Abstract

In addition to rice yellow dwarf (RYD) phytoplasma, several phytoplasmas infect gramineous plants, including rice orange leaf, bermuda grass white leaf, brachiaria grass white leaf and sugarcane white leaf phytoplasmas. To investigate whether the RYD phytoplasma is a discrete, species-level taxon, several isolates of the aforementioned phytoplasmas were analysed using PCR-amplified 16S rDNA sequences. Two RYD isolates, RYD-J and RYD-Th, were almost identical (99·2 %), but were distinct (similarities of 96·3–97·9 %) from other phytoplasma isolates of the RYD 16S-group. The notion that the RYD phytoplasma constitutes a unique taxon is also supported by its unique insect vector ( sp.), its unique host plant in nature (rice) and its limited geographical distribution (Asia). In Southern blot analysis, chromosomal and extrachromosomal DNA probes of the RYD phytoplasma reportedly did not hybridize with those of closely related phytoplasmas. These properties of the RYD phytoplasma clearly indicate that it represents a novel taxon, ‘ Phytoplasma oryzae’.

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2003-11-01
2019-12-13
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References

  1. Anonymous ( 1919; ). Annual Report 62. Japan: Kouchi Agricultural Research Institute.
  2. Anonymous ( 2002; ). Phytoplasma and Spiroplasma Working Team. In International Research Program on Comparative Mycoplasmology (IRPCM) of the 14th International Organization for Mycoplasmology (IOM) Report of Consultations, 7–12 July 2002, Vienna, Austria.
  3. Bertaccini, A., Vorácková, Z., Vibio, M., Fránová, J., Navrátil, M., Spak, J. & Nebesárová, J. ( 1998; ). Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group. Plant Pathol 47, 317–324.[CrossRef]
    [Google Scholar]
  4. Davis, R. E. & Dally, E. L. ( 2001; ). Revised subgroup classification of group 16SrV phytoplasmas and placement of flavescence dorée-associated phytoplasmas in two distinct subgroups. Plant Dis 85, 790–797.[CrossRef]
    [Google Scholar]
  5. Hibino, H., Jonson, G. B. & Sta Cruz, F. C. ( 1987; ). Association of mycoplasmalike organisms with rice orange leaf in the Philippines. Plant Dis 71, 792–794.[CrossRef]
    [Google Scholar]
  6. Jung, H.-Y., Sawayanagi, T., Kakizawa, S. & 7 other authors ( 2002; ).Candidatus Phytoplasma castaneae’, a novel phytoplasma taxon associated with chestnut witches' broom disease. Int J Syst Evol Microbiol 52, 1543–1549.[CrossRef]
    [Google Scholar]
  7. Jung, H.-Y., Sawayanagi, T., Kakizawa, S. & 7 other authors ( 2003; ).Candidatus Phytoplasma ziziphi’, a novel phytoplasma taxon associated with jujube witches'-broom disease. Int J Syst Evol Microbiol 53, 1037–1041.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Lee, I.-M., Pastore, M., Vibio, M., Danielli, A., Attathom, S., Davis, R. E. & Bertaccini, A. ( 1997; ). Detection and characterization of a phytoplasma associated with annual blue grass (Poa annua) white leaf disease in southern Italy. Eur J Plant Pathol 103, 251–254.[CrossRef]
    [Google Scholar]
  10. Nakashima, K. & Hayashi, T. ( 1995; ). Extrachromosomal DNAs of rice yellow dwarf and sugarcane white leaf phytoplasmas. Ann Phytopathol Soc Jpn 61, 456–462.[CrossRef]
    [Google Scholar]
  11. Nakashima, K. & Murata, N. ( 1993; ). Destructive plant diseases caused by mycoplasma-like organisms in Asia. Outlook Agric 22, 53–58.
    [Google Scholar]
  12. Nakashima, K., Kato, S., Iwanami, S. & Murata, N. ( 1993; ). DNA probes reveal relatedness of rice yellow dwarf mycoplasmalike organisms (MLOs) and distinguish them from other MLOs. Appl Environ Microbiol 59, 1206–1212.
    [Google Scholar]
  13. Nakashima, K., Hayashi, T., Chaleeprom, W., Wongkaew, P. & Sirithorn, P. ( 1996; ). Complex phytoplasma flora in Northeast Thailand as revealed by 16S rDNA analysis. Ann Phytopathol Soc Jpn 62, 57–60.[CrossRef]
    [Google Scholar]
  14. Namba, S., Oyaizu, H., Kato, S., Iwanami, S. & Tsuchizaki, T. ( 1993a; ). Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol 43, 461–467.[CrossRef]
    [Google Scholar]
  15. Namba, S., Kato, S., Iwanami, S., Oyaizu, H., Shiozawa, H. & Tsuchizaki, T. ( 1993b; ). Detection and differentiation of plant-pathogenic mycoplasmalike organisms using polymerase chain reaction. Phytopathology 83, 786–791.[CrossRef]
    [Google Scholar]
  16. Nasu, S., Sugiura, M., Wakimoto, T. & Iida, T. ( 1967; ). On the etiologic agent of rice yellow dwarf. Ann Phytopathol Soc Jpn 33, 343–344.
    [Google Scholar]
  17. Ou, S. H. ( 1985; ). Virus and MLO diseases. In Rice Diseases, 2nd edn, pp. 1–60. Kew, UK: Commonwealth Mycological Institute.
  18. Rivera, C. T., Ou, S. H. & Pathak, M. D. ( 1963; ). Transmission studies of the orange leaf disease of rice. Plant Dis Rep 47, 1045–1048.
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Schneider, B., Seemüller, E., Smart, C. D. & Kirkpatrick, B. C. ( 1995; ). Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology, vol. 1, pp. 369–380. Edited by S. Razin & J. G. Tully. San Diego, CA: Academic Press.
  21. Seemüller, E., Schneider, B., Mäurer, R. & 8 other authors ( 1994; ). Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA. Int J Syst Bacteriol 44, 440–446.[CrossRef]
    [Google Scholar]
  22. Seemüller, E., Marcone, C., Lauer, U., Ragozzino, A. & Göschl, M. ( 1998; ). Current status of molecular classification of the phytoplasmas. J Plant Pathol 80, 3–26.
    [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  24. Tran-Nguyen, L., Blanche, K. R., Egan, B. & Gibb, K. S. ( 2000; ). Diversity of phytoplasmas in northern Australian sugarcane and other grasses. Plant Pathol 49, 666–679.[CrossRef]
    [Google Scholar]
  25. Viswanathan, K. & Kalode, M. B. ( 1986; ). Host specificity of rice green leafhoppers, Nephotettix virescens (Distant) and Nephotettix nigropictus (Stål). Proc Anim Sci Indian Acad Sci 95, 227–236.[CrossRef]
    [Google Scholar]
  26. Wongkaew, P., Hanboonsong, Y., Sirithorn, P., Choosai, C., Boonkrong, S., Tinnangwattana, T., Kitchareonpanya, R. & Damak, S. ( 1997; ). Differentiation of phytoplasmas associated with sugarcane and gramineous weed white leaf disease and sugarcane grassy shoot disease by RFLP and sequencing. Theor Appl Genet 95, 660–663.[CrossRef]
    [Google Scholar]
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