1887

Abstract

Strain N35 was isolated from surface-sterilized wheat roots and is a Gram-negative, aerobic, motile straight rod. Strain N35 tested oxidase-positive and catalase-negative and grew optimally at pH 7.0, 30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis showed over 97 % sequence similarity to strains of the environmental species , , , , and , as well as , and . DNA–DNA hybridization between strain N35 and phylogenetically closely related type strains was 25.3–55.7 %, which clearly separates the strain from these closely related species. Additionally, phenotypic properties, such as substrate metabolism profiles as determined by a Biolog GN2 assay and cell-wall fatty acid profiles, particularly contents of the fatty acids C, Cω7/, C, C cyclo, C cyclo and C cyclo, facilitated the differentiation of the newly isolated strain N35 from its closest relatives. The isolate underwent phenotypic variation at high frequency in laboratory media. The DNA G+C content was 64.9 mol%. We propose that strain N35 is classified as a representative of a novel species within the genus and suggest the name sp. nov. The type strain is strain N35 ( = DSM 23535  = LMG 25767).

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2011-11-01
2024-11-08
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