1887

Abstract

A novel endophytic actinomycete, designated strain 202201, was isolated from an root collected from the mangrove reserve zone in Hainan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 202201 fell within the family . The strain formed an extensively branched substrate mycelium, which carried uneven warty-surfaced spores. Cell walls of strain 202201 contained -diaminopimelic acid and xylose, mannose, arabinose, ribose and glucose were detected as whole-cell sugars. The acyl type of the cell-wall polysaccharides was glycolyl. The major menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-10(H). The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylserine. The major cellular fatty acids were 10-methyl-C, iso-C, iso-C and Cω8. The DNA G+C content was 72.3 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain 202201 ( = CGMCC 4.5597 = DSM 45430) represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed.

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2011-05-01
2020-01-21
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Neighbour-joining phylogenetic tree derived from nearly complete 16S rRNA gene sequences showing the relationships between strain 202201 , 161111 and 161612 and all other recognized type strains in the family . [PDF](32kb)

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