1887

Abstract

A nitrogen-fixing bacterium, designated strain Be17, was isolated from rhizosphere soil of planted in Beijing Botanical Garden, PR China. Phylogenetic analyses based on a segment of the gene sequence and a full-length 16S rRNA gene sequence revealed that strain Be17 was a member of the genus . High levels of 16S rRNA gene sequence similarity were found between strain Be17 and RSA19 (97.9 %), LMG 24727 (97.8 %), CECT 7330 (96.2 %) and DSM 13188 (96.1 %), respectively. Levels of 16S rRNA gene sequence similarity between strain Be17 and the type strains of other recognized members of the genus were below 96.0 %. However, the DNA–DNA hybridization values between strain Be17 and RSA19, LMG 24727 and CECT 7330 were 47.9 %, 38.7 % and 37.5 %, respectively. The DNA G+C content of strain Be17 was 52.9 mol%. The major fatty acid component of strain Be17 was anteiso-branched C (30.92 %). The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. On the basis of its phenotypic characteristics, 16S rRNA gene sequences, DNA G+C content, DNA–DNA relatedness, chemotaxonomic properties and gene sequence, strain Be17 represents a nitrogen-fixing strain of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Be17 ( = CGMCC 1.10239 = DSM 23019).

Funding
This study was supported by the:
  • , National ‘973’ Project , (Award 2010CB126504)
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2011-06-01
2020-07-02
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