1887

Abstract

Fourteen sucrose-positive -like isolates from fresh water and reared European eels were subjected to a polyphasic study to determine their taxonomic position. Numerical taxonomy was used to analyse phenotypic data obtained for these isolates and 43 type and reference strains representative of recognized species. The cluster (phenon 1) was defined at 81·6 % similarity ( ); this included the -like isolates, the sucrose-positive strain biogroup sobria CECT 4910 and nearly all reference strains used in the study. Four other reference strains of biogroup sobria and the type strain of were peripheral to the cluster. The supra-group ‘ biogroup sobria–’ was linked at 80·7 % similarity ( ) and was clearly segregated from the phenotypic core of the biogroup sobria species, which was related to the reference strain Popoff 224 (CECT 4835). DNA relatedness between strains grouped in the cluster (phenon 1) and CECT 4228 ranged from 70 to 100 %, but was below 50 % when DNAs from biogroup sobria CECT 4835, biogroup veronii CECT 4257 and CECT 4486 were used. In addition, DNA relatedness between peripheral biogroup sobria strains and the species (CECT 4228), (CECT 4257, CECT 4835) and (CECT 4486) was always below 54 %, as it was between the species (CECT 4486) and (CECT 4257, CECT 4835). Emendation of is proposed; this taxon now includes sucrose-positive clinical and environmental strains as well as environmental isolates that are pathogenic for fish and humans. Other new traits for this species are the ability to grow at 4–42 °C, acid production from glycerol but not from lactose, -melibiose or -raffinose, the use of -gluconate, -glutamate or -proline but not -lactate, -alanine, -arabinose or -arginine, hydrolytic activity against casein, elastin, starch and lecithin and the inability to lyse arbutin. The DNA G+C content of is also reported for the first time; it ranges from 58·1 to 61·1 mol%. On the other hand, the DNA relatedness data support the classification of peripheral reference strains of biogroup sobria outside this taxon, indicating that biogroup sobria requires further revision.

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2003-09-01
2019-09-15
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Simplified dendrogram based on the S coefficient and UPGMA clustering algorithm, showing the relationship between the 10 phenons defined at or above 90 % similarity.

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