1887

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile bacterium, designated strain G4, was isolated from a sandy beach of Taean in South Korea. Cells were ovoid rods and were catalase- and oxidase-positive. Growth of strain G4 was determined at 15–35 °C (optimum 25–30 °C) and pH 6–8 (optimum pH 6.5–7.5). Strain G4 contained Q-10 as the predominant isoprenoid quinone and Cω7 (59.0 %), C 7 11-methyl (11.3 %) and C 3-OH (9.8 %) as the major fatty acids. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified amino lipid, an unidentified phospholipid and an unidentified lipid. The DNA G+C content was 62.4 mol%. Phylogenetic and comparative analysis based on 16S rRNA gene sequences indicated that strain G4 fell within the family of and was most closely related to members of the genera , and with 95.5–96.4 % sequence similarities. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G4 represents a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is G4 ( = KACC 13706  = DSM 22007).

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2011-06-01
2019-12-06
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References

  1. Buchan A. , Collier L. S. , Neidle E. L. , Moran M. A. . ( 2000; ). Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage. . Appl Environ Microbiol 66:, 4662–4672. [CrossRef].[PubMed]
    [Google Scholar]
  2. Buchan A. , González J. M. , Moran M. A. . ( 2005; ). Overview of the marine Roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef].[PubMed]
    [Google Scholar]
  3. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef].[PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 2002; ). phylip (phylogeny inference package), version 3.6a.. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Giovannoni S. J. , Rappé M. . ( 2000; ). Evolution, diversity, and molecular ecology of marine prokaryotes. . In Microbial Ecology of the Oceans, pp. 47–84. Edited by Kirchman D. L. . . New York:: Wiley;.
    [Google Scholar]
  6. Gomori G. . ( 1955; ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1:, 138–146. [CrossRef]
    [Google Scholar]
  7. Jeon C. O. , Park W. , Ghiorse W. C. , Madsen E. L. . ( 2004; ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54:, 93–97. [CrossRef].[PubMed]
    [Google Scholar]
  8. Kahng H.-Y. , Chung B. S. , Lee D.-H. , Jung J.-S. , Park J. H. , Jeon C. O. . ( 2009; ). Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater. . Int J Syst Evol Microbiol 59:, 654–657. [CrossRef].[PubMed]
    [Google Scholar]
  9. Kim J. M. , Le N. T. , Chung B. S. , Park J. H. , Bae J.-W. , Madsen E. L. , Jeon C. O. . ( 2008; ). Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. . Appl Environ Microbiol 74:, 7313–7320. [CrossRef].[PubMed]
    [Google Scholar]
  10. Kim J. M. , Lee S. H. , Jung J. Y. , Jeon C. O. . ( 2010; ). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 60:, 1828–1831. [CrossRef].[PubMed]
    [Google Scholar]
  11. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  12. Lafay B. , Ruimy R. , de Traubenberg C. R. , Breittmayer V. , Gauthier M. J. , Christen R. . ( 1995; ). Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . . Int J Syst Bacteriol 45:, 290–296. [CrossRef].[PubMed]
    [Google Scholar]
  13. Lu S. , Park M. , Ro H.-S. , Lee D. S. , Park W. , Jeon C. O. . ( 2006; ). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44:, 155–161.[PubMed]
    [Google Scholar]
  14. Martens T. , Heidorn T. , Pukall R. , Simon M. , Tindall B. J. , Brinkhoff T. . ( 2006; ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef].[PubMed]
    [Google Scholar]
  15. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  16. Moran M. A. , Gonzalez J. M. , Kiene R. P. . ( 2003; ). Linking a bacterial taxon to sulfur cycling in the sea: studies of the marine Roseobacter group. . Geomicrobiol J 20:, 375–388. [CrossRef]
    [Google Scholar]
  17. Ruiz-Ponte C. , Cilia V. , Lambert C. , Nicolas J. L. . ( 1998; ). Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . . Int J Syst Bacteriol 48:, 537–542.[PubMed] [CrossRef]
    [Google Scholar]
  18. Schaefer J. K. , Goodwin K. D. , McDonald I. R. , Murrell J. C. , Oremland R. S. . ( 2002; ). Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. . Int J Syst Evol Microbiol 52:, 851–859. [CrossRef].[PubMed]
    [Google Scholar]
  19. Shiba T. . ( 1991; ). Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. . Syst Appl Microbiol 14:, 140–145.[CrossRef]
    [Google Scholar]
  20. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Sun F. , Wang B. , Liu X. , Lai Q. , Du Y. , Li G. , Luo J. , Shao Z. . ( 2010; ). Leisingera nanhaiensis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60:, 275–280. [CrossRef].[PubMed]
    [Google Scholar]
  22. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  23. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef].[PubMed]
    [Google Scholar]
  24. Vandecandelaere I. , Segaert E. , Mollica A. , Faimali M. , Vandamme P. . ( 2008; ). Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. . Int J Syst Evol Microbiol 58:, 2788–2793. [CrossRef].[PubMed]
    [Google Scholar]
  25. Vandecandelaere I. , Segaert E. , Mollica A. , Faimali M. , Vandamme P. . ( 2009; ). Phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm. . Int J Syst Evol Microbiol 59:, 1209–1214. [CrossRef].[PubMed]
    [Google Scholar]
  26. Wang Q. , Garrity G. M. , Tiedje J. M. , Cole J. R. . ( 2007; ). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol 73:, 5261–5267. [CrossRef].[PubMed]
    [Google Scholar]
  27. Yoon J. H. , Kang S. J. , Lee S. Y. , Oh T. K. . ( 2007; ). Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 57:, 856–861. [CrossRef].[PubMed]
    [Google Scholar]
  28. Zhang D. C. , Li H. R. , Xin Y. H. , Liu H. C. , Chi Z. M. , Zhou P. J. , Yu Y. . ( 2008; ). Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. . Int J Syst Evol Microbiol 58:, 1384–1387. [CrossRef].[PubMed]
    [Google Scholar]
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