1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 6

gen. nov., sp. nov., isolated from a sandy beach Free

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile bacterium, designated strain G4, was isolated from a sandy beach of Taean in South Korea. Cells were ovoid rods and were catalase- and oxidase-positive. Growth of strain G4 was determined at 15–35 °C (optimum 25–30 °C) and pH 6–8 (optimum pH 6.5–7.5). Strain G4 contained Q-10 as the predominant isoprenoid quinone and Cω7 (59.0 %), C 7 11-methyl (11.3 %) and C 3-OH (9.8 %) as the major fatty acids. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified amino lipid, an unidentified phospholipid and an unidentified lipid. The DNA G+C content was 62.4 mol%. Phylogenetic and comparative analysis based on 16S rRNA gene sequences indicated that strain G4 fell within the family of and was most closely related to members of the genera , and with 95.5–96.4 % sequence similarities. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G4 represents a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is G4 ( = KACC 13706  = DSM 22007).

  • Published Online:
Funding
This study was supported by the:
  • MOST/KOSEF (Award MG05-0104-4-0)
  • National Research Foundation of Korea (Award 2009-0071880)
  • Korean Government (MEST)
© 2011 IUMS

Erratum

An erratum has been published for this content:
gen. nov., sp. nov., isolated from a sandy beach
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025007-0
2011-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/6/1392.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025007-0&mimeType=html&fmt=ahah

References

  1. Buchan A., Collier L. S., Neidle E. L., Moran M. A. 2000; Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage. Appl Environ Microbiol 66:4662–4672 [View Article][PubMed]
     [Google Scholar]
  2. Buchan A., González J. M., Moran M. A. 2005; Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71:5665–5677 [View Article][PubMed]
     [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  4. Felsenstein J. 2002; phylip (phylogeny inference package), version 3.6a.. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  5. Giovannoni S. J., Rappé M. 2000; Evolution, diversity, and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans pp. 47–84 Edited by Kirchman D. L. New York: Wiley;
     [Google Scholar]
  6. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146 [View Article]
     [Google Scholar]
  7. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. 2004; Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54:93–97 [View Article][PubMed]
     [Google Scholar]
  8. Kahng H.-Y., Chung B. S., Lee D.-H., Jung J.-S., Park J. H., Jeon C. O. 2009; Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater. Int J Syst Evol Microbiol 59:654–657 [View Article][PubMed]
     [Google Scholar]
  9. Kim J. M., Le N. T., Chung B. S., Park J. H., Bae J.-W., Madsen E. L., Jeon C. O. 2008; Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74:7313–7320 [View Article][PubMed]
     [Google Scholar]
  10. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O. 2010; Marinobacterium lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 60:1828–1831 [View Article][PubMed]
     [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  12. Lafay B., Ruimy R., de Traubenberg C. R., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [View Article][PubMed]
     [Google Scholar]
  13. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. 2006; Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44:155–161[PubMed]
     [Google Scholar]
  14. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. 2006; Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . Int J Syst Evol Microbiol 56:1293–1304 [View Article][PubMed]
     [Google Scholar]
  15. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
     [Google Scholar]
  16. Moran M. A., Gonzalez J. M., Kiene R. P. 2003; Linking a bacterial taxon to sulfur cycling in the sea: studies of the marine Roseobacter group. Geomicrobiol J 20:375–388 [View Article]
     [Google Scholar]
  17. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. 1998; Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48:537–542[PubMed] [CrossRef]
     [Google Scholar]
  18. Schaefer J. K., Goodwin K. D., McDonald I. R., Murrell J. C., Oremland R. S. 2002; Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. Int J Syst Evol Microbiol 52:851–859 [View Article][PubMed]
     [Google Scholar]
  19. Shiba T. 1991; Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14:140–145 [CrossRef]
     [Google Scholar]
  20. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  21. Sun F., Wang B., Liu X., Lai Q., Du Y., Li G., Luo J., Shao Z. 2010; Leisingera nanhaiensis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 60:275–280 [View Article][PubMed]
     [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  24. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P. 2008; Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. Int J Syst Evol Microbiol 58:2788–2793 [View Article][PubMed]
     [Google Scholar]
  25. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P. 2009; Phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59:1209–1214 [View Article][PubMed]
     [Google Scholar]
  26. Wang Q., Garrity G. M., Tiedje J. M., Cole J. R. 2007; Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267 [View Article][PubMed]
     [Google Scholar]
  27. Yoon J. H., Kang S. J., Lee S. Y., Oh T. K. 2007; Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57:856–861 [View Article][PubMed]
     [Google Scholar]
  28. Zhang D. C., Li H. R., Xin Y. H., Liu H. C., Chi Z. M., Zhou P. J., Yu Y. 2008; Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1384–1387 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025007-0
Loading
Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach
Int J Syst Evol Microbiol 61, 1392 (2011); https://doi.org/10.1099/ijs.0.025007-0
/content/journal/ijsem/10.1099/ijs.0.025007-0
/content/journal/ijsem/10.1099/ijs.0.025007-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error