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Abstract

A novel Gram-negative, non-motile bacterium, designated strain ZS2-28, was isolated from sandy intertidal sediment samples collected from the coastal regions of the Chinese Antarctic Zhongshan Station on the Larsemann Hills, Princess Elizabeth Land, East Antarctica. Strain ZS2-28 was obligately heterotrophic, strictly aerobic, psychrotolerant (growth occurred at 0–33 °C) and moderately halophilic (optimal growth in 7–8 % NaCl). A single major peak at 872–874 nm in the infrared absorption spectrum indicated the presence of bacteriochlorophyll . Poly-β-hydroxybutyrate accumulation and slime production were also detected. The predominant cellular fatty acid was Cω7, with C 3-OH, C, C cyclo, Cω8 cyclo and summed feature 3 (Cω7 and/or iso-C 2-OH) present in smaller amounts. The respiratory quinone was Q-10. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminolipid. The G+C content of the genomic DNA was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZS2-28 formed a distinct evolutionary lineage within the clade containing members of the genera , and of the class . On the basis of its phylogenetic position, as well as its phenotypic and chemotaxonomic characteristics, strain ZS2-28 represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is ZS2-28 ( = CGMCC 1.8894  = LMG 24863).

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2011-09-01
2019-10-18
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vol. , part 9, pp. 2173 - 2179

Transmission electron micrograph of strain ZS2-28 .

absorption spectrum of strain ZS2-28 .

Cross-section transmission electron micrograph of strain ZS2-28 .

Thin-layer chromatogram of polar lipids from strain ZS2-28 .

Phylogenetic dendrogram of and related species based on 16S rRNA sequences and reconstructed using the maximum-parsimony method.

Phylogenetic dendrogram of and related species based on 16S rRNA sequences and reconstructed using the neighbour-joining method.

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