1887

Abstract

The taxonomic position of a group of five -sorbitol- and lactose-negative enterobacterial isolates recovered from diarrhoeal stools of children at the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), was investigated by DNA–DNA hybridization, phenotypic characterization and 16S rDNA sequencing. These strains were originally identified as ‘-like’ with the API 20E system but, in fact, show more phenotypic and genotypic resemblance to members of the genus . By 16S rDNA sequencing, one representative strain of the ICDDR,B group was shown to be closely affiliated to the genera and . Using the fluorimetric microplate hybridization method, the diarrhoeagenic ICDDR,B isolates were found to constitute a homogeneous taxon (⩾82 % internal DNA relatedness), with the closest affiliation to the type strains of (55–64 %) and (54–60 %). The DNA–DNA hybridization levels were much lower with members of other described species (16–45 %) and with the type strain of (9–17 %). The G+C content of the ICDDR,B strains ranged from 50·5 to 50·7 mol%. Together with the diagnostic characteristics reported previously, including the presence of the gene of enteropathogenic and of the and -specific gene, it is concluded that the ICDDR,B strains represent a novel taxon in the genus , for which the name sp. nov. is proposed. Its type strain is Albert 19982 (=LMG 20976=CCUG 46494).

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2003-05-01
2019-09-24
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