1887

Abstract

A psychrotolerant, facultatively alkaliphilic strain, HT-3, was isolated from a sample of soil immersed in hot-spring water containing hydrocarbons in Toyotomi, Hokkaido, Japan. 16S rRNA gene sequence-based phylogeny suggested that strain HT-3 is a member of the genus and belongs to the group. Cells of the isolate were Gram-negative, aerobic, straight rods, motile by a single polar flagellum. The strain grew at 4–42 °C, with optimum growth at 35 °C at pH 7, and at pH 6–10. It hydrolysed Tweens 20, 40, 60 and 80, but not casein, gelatin, starch or DNA. Its major isoprenoid quinone was ubiquinone-9 (Q-9) and the DNA G+C content was 65.1 mol%. The whole-cell fatty acid profile consisted mainly of C, Cω9 and Cω9. Phylogenetic analyses based on , and sequences revealed that the isolate could be discriminated from species that exhibited more than 97 % 16S rRNA gene sequence similarity and phylogenetic neighbours belonging to the group including the closest relative of the isolate, . DNA–DNA hybridization with . AL15-21 revealed 51±5 % relatedness. Owing to differences in phenotypic properties and phylogenetic analyses based on multilocus gene sequence analysis and DNA–DNA relatedness data, the isolate merits classification in a novel species, for which the name sp. nov. is proposed. The type strain is HT-3 ( = JCM 15604  = NCIMB 14511).

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2011-08-01
2019-10-20
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Transmission electron micrograph of negatively stained cells of sp. nov. HT-3 . Bar, 1 µm.

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