1887

Abstract

A rod shaped, Gram-stain-negative, chemo-organotrophic, heterotrophic, strictly aerobic, non-gliding bacterium, designated strain PLR, was isolated from faeces of the mollusc (Mollusca, Gastropoda) that had been fed with green algae belonging to the genus . The novel strain was able to degrade ulvan, a polysaccharide extracted from green algae (Chlorophyta, Ulvophyceae). The taxonomic position of strain PLR was investigated by using a polyphasic approach. Strain PLR was dark orange, oxidase-positive, catalase-positive and grew optimally at 25 °C, at pH 7.5 and in the presence of 2.5 % (w/v) NaCl with an oxidative metabolism using oxygen as the electron acceptor. Nitrate could not be used as the electron acceptor. Strain PLR had a Chargaff’s coefficient (DNA G+C content) of 35.3 mol%. Phylogenetic analysis based on the sequence of the 16S rRNA gene placed the novel strain in the family (phylum ‘’), within a clade comprising , , , and . The closest neighbours of strain PLR were and , sharing 95.2 and 95.5 % 16S rRNA gene sequence similarity, respectively. Phylogenetic inference and differential phenotypic characteristics demonstrated that strain PLR represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PLR ( = CIP 110082 = DSM 22727).

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2011-08-01
2019-10-16
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SDS-PAGE of protein extracts from strain PLR (lane 1), SW256 (lane 2) and DSW-6 (lane 3). Protein bands were visualized by staining with Coomassie blue.

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Polar lipid profile of strain PLR (a), SW256 (b) and DSW-6 (c), after two-dimensional TLC and detection with 5% molybdophosphoric acid. PE, phosphatidylethanolamine; PL, phospholipids; L1–L4, unidentified lipids; AL1–AL2, unidentified aminolipids.

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